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论文摘要

双因子诱导轴型支架血管化的组织形态学研究

Histological study of dual factor inducing axial vascularization in double-layered scaffold

作者:李受益 蔡庆义 陈刚 朱晓虹 钟晓敏 彭德瑞

Author:Li Shouyi, Cai Qingyi, Chen Gang, Zhu Xiaokong, Zhong Xiaomin, Peng Derui

收稿日期:          年卷(期)页码:2013,31(2):205-205-208

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:骨组织工程,血管化,动静脉血管束,血管新生,

Key words:bone tissue engineering,vascularization,arteriovenous bundle,angiogenesis,

基金项目:

教育部留学回国人员科研启动基金资助项目[教外司留 (2012) 940]

中文摘要

目的 以血管内皮生长因子(VEGF)和血小板衍生生长因子(PDGF)复合同轴双层聚乳酸-羟基乙酸共聚物(PLGA)支架并包裹股动静脉血管束,观察血管再生与成熟及支架早期血管化的动态变化。方法 将18只3月龄成年雄性新西兰大耳兔随机分为3组:实验组(8只)、实验对照组(8只)和空白对照组(2只)。解剖分离兔的股动静脉血管束,将其包裹于溶液浇铸-颗粒沥取法制备的同轴双层PLGA支架中央。实验组支架的外层加注PDGF,内层加注 VEGF;实验对照组支架的外层为空白,内层加注VEGF;空白对照组不予特殊处理。实验组及实验对照组于支架植入术后第7、10、14、21天观察新生血管的形态特征,空白对照组于支架植入术后第7、10天观察作为对照。结果 术后第7天,实验组及实验对照组股动静脉血管束可见少量呈放射状分布的血管芽;术后第10天,两组大量的新生血管芽沿支架孔隙空间向内层支架放射状生长并贯通支架全层,密度由内向外梯度递减;术后第14天,实验组血管壁较厚,呈分层结构,实验对照组以单层内皮样细胞衬里的幼稚血管为主;术后第21天,实验组支架内以成熟血管为 主,而实验对照组血管数量明显减少。空白对照组始终未见明显血管结构。结论 同轴双层PLGA支架复合VEGF/ PDGF可有效促进早期血管化。

英文摘要

Objective To construct an double-layered tube-shaped poly (lactic-co-glycolic acid) (PLGA) scaffold composited with vascular endothelial growth factor (VEGF) / platelet-derived growth factor (PDGF) , and to evaluate in vivo the axial vascularization from femoral arteriovenous bundle encapsulated.Methods Eighteen male adult New Zealand rabbits were assigned randomly into 3 groups: Experimental group (n=8) , experimental control group (n =8), empty control group (n=2). The femoral arteriovenous bundle were separated and encapsulated in double-layered tubeshaped PLGA scaffold prepared by solution casting and particle leaching. According to the way of cell factors being composited to the prepared double-layered scaffold: 1) experimental group: VEGF in inner layer and PDGF in outer layer; 2) experimental control group: VEGF in inner layer with blank outer layer; 3) empty control group: Pure blank. Specimens were retrieved at 7, 10, 14, 21 days postoperatively (7, 10 days in empty control group) . The histological evaluation was performed.Results At 7 days postoperatively, blood vessel sprouts were observed in experimental group and experimental control group, radially from the central femoral arteriovenous bundle. At 10 days postoperatively, completely penetrations of the double-layered scaffold by abundant new generated blood vessel sprouts were observed, and density descended gradiently from inside to outside. At 14 days postoperatively, new blood vessels in experimental group showed more thickness and layered structure of the wall, while monolayer endothelial cells in experimental control group. At 21 days postoperatively, new blood vessels in experimental group showed more mature characteristics,while less density of blood vessels in experimental control group. There was no obvious blood vessel structure in empty control group.Conclusion The double -layered tube-shaped PLGA scaffold composited with VEGF/PDGF could induce early angiogenesis.

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