人β防御素-4在牙龈组织表达的新发现
New finding of the expression of human β defensin-4 in healthy gingiva
作者：李欣忆 段丁瑜 王盼盼 韩波 徐屹
Author：Li Xinyi, Duan Dingyu, Wang Panpan, Han Bo, Xu Yi
收稿日期： 年卷（期）页码：2013,31(2):165-165-168
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙龈,人β防御素-4,免疫组织化学,实时荧光定量逆转录聚合酶链反应,
Key words：gingiva,human &beta,defensin -4,immunohistochemistry,real time reverse transcription-polymerase chain reaction,
基金项目：
国家自然科学基金资助项目（30872872）；四川省科技厅科技支撑计划基金资助项目（2011FZ0044）
中文摘要
目的 探讨牙龈组织中是否存在β防御素-4（hBD-4）及其分布情况。方法 收集健康牙龈标本。采用免疫组织化学法检测18例牙龈组织标本hBD-4蛋白水平的分布；提取组织RNA，进行实时荧光定量逆转录聚合酶链反应（real time RT-PCR）检测30例牙龈标本中hBD-4基因的表达水平。结果 免疫组织化学检测结果显示，18例牙龈样本中有13例检出hBD-4；real time RT-PCR检测结果显示，30例牙龈组织中4例样本检测出hBD-4表达。结论 hBD-4在健康牙龈组织中有不同水平的表达和分布，可能在维持牙周组织健康中起作用。
英文摘要
Objective To investigate the expression and the distribution of human β defensin (hBD)-4 in healthy gingiva.Methods Healthy gingival specimens were collected. The expression of hBD-4 peptides in 18 gingival specimens were detected by immunohistochemistry. The hBD-4 mRNA were determined in freshly isolated gingival tissue by real time reverse transcription-polymerase chain reaction (real time RT-PCR) in 30 gingival specimens.Results In 18 gingival specimens, hBD-4 peptides were expressed in 13 gingival specimens. In 30 gingival specimens, hBD-4 were detected in 4 gingival specimens by real time RT-PCR.Conclusion The distribution and the expression levels of hBD-4 are different in healthy gingiva. This result may suggest that the hBD-4 play a role in maintaining the periodontal health.
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