纳米抗菌复合膜的结构及作为引导骨组织再生膜的生物相容性研究
Research on the micro structure of antibacterial nanocomposite membrane and it’s biocompatibility as a guided bone regeneration membrane
作者：刘曼 张强 周立伟 莫安春 李小玉 李吉东3
Author：Liu Man, Zhang Qiang, Zhou Liwei, Mo Anchun, Li Xiaoyu, Li Jidong
收稿日期： 年卷（期）页码：2013,31(2):127-127-130
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：引导骨再生,羟磷灰石,银,成骨样细胞MG63,
Key words：guided bone regeneration,hydroxyapatite,silver,MG63 osteoblast-like cells,
基金项目：
中文摘要
目的 观察载银纳米羟磷灰石/二氧化钛/聚酰胺66（Ag-nHA-nTiO2/PA66）纳米抗菌复合膜的物理结构及对成骨样细胞生物相容性的影响。方法 扫描电镜（SEM）和光学显微镜下观察Ag-nHA-nTiO2/PA66膜和膨体聚四氟乙烯（e-PTFE）膜的结构，在两组膜材料上接种成骨样细胞MG63，另设空白对照组。甲基噻唑基四唑（MTT）法检测MG63细 胞的增殖曲线，酶联免疫法检测细胞内碱性磷酸酶（ALP）的表达，SEM下观察细胞在生物膜表面的增殖和黏附情况。结果 Ag-nHA-nTiO2/PA66膜正面疏松多孔，反面光滑致密。e-PTFE膜正反面结构相同，由成行排列的长椭圆形裂隙组成。与空白对照组相比，MTT检测显示，两种膜对MG63细胞增殖活力差异无统计学意义（P>0.05）；ALP活性间差异也无统计学意义（P>0.05）。SEM下观察细胞在两种膜上生长良好，在Ag-nHA-nTiO2/PA66膜上细胞伸展更充分。 结论 Ag-nHA-nTiO2/PA66膜对MG63细胞生长无抑制作用。与e-PTFE相比，其具有更优良的结构和生物学性能， 适于用作引导骨再生膜材料。
英文摘要
Objective To study the microstructure of the Ag-nHA-nTiO2/PA66 membrane and investigate its biocompatibility.Methods The microstructure of Ag-nHA-nTiO2/PA66 membrane and e-polytetra fluoroethylene (e-PTFE) membrane were observed by light microscope and scanning electron microscope (SEM) . MG63 osteoblast-like cells were cultured on the two kinds of membrane and blank group. The cell proliferation was checked by methyl thiazolyl te- trazolium (MTT) method and alkaline phosphatase (ALP) activity was detected by enzyme linked immunosorbent assay (ELISA) . The adhesion and proliferation of the cells on the two kinds of membrane was observed by SEM.Results The Ag-nHA-nTiO2/PA66 membrane was composed of the obverse face and the opposite face. The obverse face was porous and the opposite face was smooth. Microstructures of the obverse and the opposite face of the e-PTFE membrane were same. The e-PTFE membrane showed many tiny lined cracks in elliptic structure. MTT assay and ALP measurement showed that there were no significant difference between each of the two membrane groups and the blank (P>0.05). The adhesion and proliferation of cells on the Ag-nHA-nTiO2/PA66 membrane were better than the e-PTFE membrane.Conclusion Ag-nHA-nTiO2/PA66 membrane has no negative effects on the growth of osteoblast-like cells. Ag-nHA-nTiO2/PA66 membrane is biocompatible and its microstructure is appropriate as a guided bone regeneration materials.
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