Objective To evaluate the effect of concentrated growth factor extract (CGFe) on the proliferation and differentiation of MC3T3-E1 osteoblasts attached to sandblasted and acid etched titanium surfaces.MethodsTrials were divided into experimental and control groups. The experimental group used α-MEM that contained CGFe (10% FBS), whereas the control group only used α-MEM (10% FBS). MTT assay was employed to detect the number of osteoblasts on the first, third, and fifth days. Alkaline phosphatase (ALP) activity and scanning electron microscope (SEM) were used to detect the osteoblast differentiations on the third and fifth days and to observe the osteoblast extensions on titanium surfaces for 12 h, respectively. Meanwhile, the levels of the osteogenetic biomarkers Runt-related transcription factor-2 (Runx2) and Osterix (Osx) on the third and seventh days were quantified via real-time polymerase chain reaction (PCR).Results MTT assay indicated that on the first, third, and fifth days, the absorbance in the experimental group significantly increased than that in the control group (PConclusion CGFe can efficiently stimulate the proliferation, differentiation and extension of MC3T3-E1 cells.
