微小RNA-17在糖基化终末产物刺激下人牙周膜干细胞骨向分化过程中的调控作用
Effect of microRNA-17 on osteogenic differentiation of advanced glycation end products-stimulated human periodontal ligament stem cells
作者：邓超1 伍燕1 杨琨1 崔晓霞1 刘琪1 金岩2
Author：Deng Chao1, Wu Yan1, Yang Kun1, Cui Xiaoxia1, Liu Qi1, Jin Yan2
收稿日期： 年卷（期）页码：2015,33(1):21-21-24
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字： ,糖基化终末产物,人牙周膜干细胞,微小RNA-17,
Key words：advanced glycation end products, human periodontal ligament stem cells,microRNA-17,
基金项目：
国家自然科学基金资助项目（81360168）；皖南医学院重点科研项目培育基金资助项目（WK2013Z10）
中文摘要
目的 检测微小RNA-17（mir-17）在糖基化终末产物（AGEs）刺激下人牙周膜干细胞（HPDLSCs）骨向分化过程中的表达，并分析其对该过程的影响。方法 体外组织块法和有限稀释法克隆化培养HPDLSCs。实时定量聚合酶链反应（real time PCR）检测实验组细胞在骨向分化过程中不同时间点mir-17的表达；采用细胞转染技术过表达和抑制mir-17的表达，real time PCR和Western blot分别检测转染前后其成骨基因mRNA水平和蛋白水平的表达情况。结果 成骨诱导3、7、14 d后，对照组和实验组mir-17的表达均下调，差异有统计学意义（P结论 AGEs通过影响HPDLSCs骨向分化过程中mir-17的表达从而抑制了HPDLSCs的骨向分化。
英文摘要
Objective This study aims to detect microRNA-17(mir-17) expression on the osteogenic differentiation of advanced glycation end products (AGEs)-stimulated hunman periodontal ligament stem cells (HPDLSCs) and to analyze the influence of these cells on this process.Methods HPDLSCs were isolated using limited dilution technique. After osteogenic differentiation occurred, different time points of mir-17 expression in the experimental groups were detected by real time polymerase chain reaction (PCR). The mir-17 overexpression and inhibition were evaluated using cell transfection technique. Differences in gene expressions were detected by real time PCR; differences in protein expressions were analyzed by Western blot.Results The mir-17 expression was reduced after osteogenic differentiation occurred at 3, 7, and 14 d compared with that in the control group (PConclusion AGEs inhibit the osteogenic differentiation of HPDLSCs by affecting mir-17 expression.
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