Objective In situ, real timely and dynamically monitoring the processes of theaflavin（TF）, curcumin （Cur）and cyanidin（Cy）binding to human whole saliva（WS）surface has been investigated by surface plasmon resonance （SPR） technique at the molecular level. Methods The affinity between pigments and WS, association rate constant （ka ） , dissociation rate constant（kd）, association equilibrium constant（KA） and dissociation equilibrium constant（KD） of
pigments binding on WS surface had been determined by SPR and the Langmuir model as well as the Freundlich model. The data were analyzed by one-way ANOVA and SNK-q test. Results There were significant differences among TF, Cur and Cy in ka, kd, KA and KD（PCur>Cy（P
