Objective To detect the ability of Enterococcus faecalis（E.faecalis） biofilm formation and explore the relationship between E.faecalis biofilm formation ability and clinical manifestation. Methods 96 well plate with the establishment of 53 E.faecalis in vitro biofilm model, combined with crystal violet staining, was used to test the biofilm formation ability of the clinical isolates E.faecalis and analyze the relationship between biofilm formation capacity and clinical manifestation. Results In total 53 E.faecalis strains, 40 strains（75.47%） had biofilm forming ability. Statistical analysis revealed that the capacities of biofilm formation between E.faecalis isolated from with fistula and without fistula was significantly different（P
