Objective To construct strains containing green fluorescent protein（GFP） to study gene regulation in Saccharomyces albicans cells during the infection process. Methods pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy. Results 99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference. Conclusion pACT1-GFP can be expressed stably in the yeast cells.
