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论文摘要

少突胶质细胞系基因-髓鞘碱性蛋白在口腔扁平苔藓中表达的研究

The progress in expression of genes of the oligodendrocyte lineage-myelin basic protein in oral lichen planus

作者:曾娟 许隽永 范媛

Author:Zeng Juan1,2, Xu Juanyong1, Fan Yuan1

收稿日期:2011-12-25          年卷(期)页码:2011,29(06):576-576-579

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:口腔扁平苔藓,少突胶质细胞系基因-髓鞘碱性蛋白,外周血单个核细胞,

Key words:oral lichen planus,genes of the oligodendrocyte lineage-myelin basic protein,peripheral blood mononuclear cell,

基金项目:

国家自然科学基金资助项目(81170961);南京医科大学科技发展基金重点项目资助项目(06NMUZO22);江苏高校优势学科建设工程资助项目(2011年)

中文摘要

目的通过观察少突胶质细胞系基因-髓鞘碱性蛋白(Golli-MBP)在口腔扁平苔藓(OLP)患者外周血单个核细胞中的表达,探索OLP的发病机制。方法采用密度梯度离心法分离20例充血糜烂型、16例光滑型OLP患者,以及19例对照组的外周血单个核细胞,分别用逆转录-聚合酶链反应(RT-PCR)法和Western blot法检测3组研究对象外周血单个核细胞中Golli-MBP mRNA和蛋白的表达。结果充血糜烂型和光滑型OLP患者中Golli-MBP mRNA和蛋白的表达水平均高于对照组,且差异有统计学意义(P0.05)。结论Golli-MBP在OLP的发病过程中起着重要作用。

英文摘要

Objective The aim of this study was to determine the expression of genes of the oligodendrocyte lineagemyelin basic protein(Golli-MBP) in peripheral blood mononuclear cell(PBMC) in oral lichen planus(OLP) and to further understand the pathogenesis of OLP. Methods PBMC was obtained by density gradient centrifugation, and the expression of Golli-MBP in PBMC was investigated in erythematous/erosive OLP(20 cases), reticular OLP(16 cases) and normal controls(19 cases) using reverse transcription-polymerase chain reaction(RT-PCR) and Western blot methods. Results RT-PCR results showed that Golli-MBP mRNA was overexpressed in erythematous/erosive and reticular OLP as compared with normal control group(P0.05). Conclusion The data accumulated here strongly indicate that Golli-MBP was involved in the pathogenesis of OLP.

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