骨形态发生蛋白-2基因转染对舌癌细胞增殖活性的影响
Effects of bone morphogenetic protein-2 on proliferation of tongue cancer Tca8113
作者：姜娟 张君 王旭霞 薛立伟 宋洪宁 张茜
Author：Jiang Juan1, Zhang Jun1, Wang Xuxia1, Xue Liwei1，2, Song Hongning1, Zhang Xi1
收稿日期：2011-10-25 年卷（期）页码：2011,29(05):546-546-549
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：骨形态发生蛋白-2,腺病毒,基因转染,舌癌细胞,
Key words：bone morphogenetic protein-2,adenovirus,gene transfection,tongue cancer cells,
基金项目：
山东省科学技术发展计划基金资助项目（2010GSF10239）
中文摘要
目的探讨腺病毒介导的人骨形态发生蛋白-2（BMP-2）基因转染舌癌Tca8113细胞后，对Tca8113细胞形态和增殖活性的影响。方法分别以感染复数（MOI）为0、50、100的腺病毒为载体的BMP-2基因转染体外培养的舌癌Tca8113细胞。采用荧光倒置显微镜观察转染前后Tca8113细胞形态的变化，Western blot法检测Tca8113细胞BMP-2的表达，四甲基偶氮唑盐（MTT）法检测Tca8113细胞增殖能力，并绘制生长曲线。结果MOI为100时转染率最高，转染前后Tca8113细胞形态变化不大，Western blot检测到Tca8113细胞BMP-2的表达，转染后Tca8113细胞的增殖活性降低。结论腺病毒介导的人BMP-2在体外能够抑制舌癌Tca8113细胞的增殖活性。
英文摘要
Objective To investigate the morphology and proliferation effects of adenovirus containing bone morphogenetic protein-2（BMP-2） on tongue cancer Tca8113 cells. Methods Tca8113 cells were transfected with the Ad- BMP-2 of 0, 50, 100 multiplicity of infection（MOI） respectively. Inverted fluorescence microscope was used to evaluate the morphological changes of these cells. Western blot analysis was performed to determine the expression levels of BMP-2 in the transfected cells. Methyl thiazolyl tetrazolium（MTT） assay was performed to monitor the proliferative activity of the infected Tca8113 cells and then the growth curve was made. Results The transfection efficiency reached the highest when the MOI was 100. Moreover, the expression of BMP-2 was detected in Tca8113 cells by Western blot. There were no obvious morphological changes of the Tca8113 cells before and after transfection. And the proliferation of ransfected Tca8113 cells decreased compared with control. Conclusion Ad-BMP-2 gene can inhibit the proliferation of Tca8113 cells in vitro.
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