偏侧咀嚼致咀嚼肌功能紊乱发病机制的研究
Research about the mechanism in masticatory muscle dysfunctional induced by hemimastication
作者：王子娴 徐龙博 祁冬 林雪芬 应王贵 孙圣军 陈彬 汲平
Author：WANG Zixian1, XU Long-bo1, QI Dong1, LIN Xue-fen1, YING Wang-gui1, SUN Sheng-jun1, CHEN Bin1, JI Ping1,2
收稿日期：2011-02-25 年卷（期）页码：2011,29(01):96-96-99
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：偏侧咀嚼,钙调神经磷酸酶,肌纤维类型,肌功能紊乱,
Key words：hemimastication,calcinuerin,muscle fiber type,masticatory muscles dysfunction,
基金项目：
山东省自然科学基金资助项目（Y2006c216）
中文摘要
目的探讨偏侧咀嚼所导致的咀嚼肌功能紊乱的发生机制。方法用原子分光光度法检测Ca2+含量；用比色法检测钙调神经磷酸酶活性；用三磷酸腺苷酶（ATPase）染色检测肌纤维类型。结果1）除8周组外，实验组拔牙侧Ca2+含量均升高，且明显高于对照组（P＜0.05）。其中，4周组Ca2+含量升高最为明显。2）实验组非拔牙侧Ⅰ型肌纤维/（Ⅰ+Ⅱ）型肌纤维比例升高，高于正常对照组水平（P＜0.05）。3）CaN活性随Ca2+含量的升高呈钟形变化。4）慢肌纤维比例与CaN活性呈正相关（r=0.876，P＜0.05）。结论高含量Ca2+激活了与骨骼肌生长和肌纤维转化有关的信号通路，使肌纤维实现从快到慢的转化。这可能是偏侧咀嚼致咀嚼肌功能紊乱的发生机制之一。
英文摘要
Objective To study the mechanism in masticatory muscle dysfunctional induced by hemimastication. Methods Ca2 + contents were measured with atomic absorption spectrometry; calcinuerin were measured with colorimetric method; muscle fiber types were measured with adenosine-triphosphate（ATPase） staining. Results 1） Compared with the controls, Ca2+ contents in experimental group had the higher level except 8 weeks（P＜0.05）. 2） The ratio of slow fiber in experimental group increased, higher than the match groups（P＜0.05）. 3）With Ca2+ contents rise， the activities of calcinuerin present a bell -like shape. 4）The ratio of slow -type fiber was positively correlated to the activities of calcinuerin（r=0.876，P＜0.05）. Conclusion The signal way of muscle fiber growth and fiber transformation were activated by high concentration of calcium，then，muscle fiber transfered from fast to slow type. It may play an important role in the mechanism that hemimastication result in masticatory muscles dysfunction.
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