苦参碱对腺样囊性癌细胞周期阻滞和端粒酶催化亚基表达的影响
Effect of Matrine on cell cycle and human telomerase reverse transcriptase of human salivary adenoid cystic carcinoma
作者：赵军方 谢伟红 李新明 陈万涛 孙明磊 方政 孙强
Author：ZHAO Jun-fang1, XIE Wei-hong1, LI Xin-ming1, CHEN Wan-tao2, SUN Ming-lei1, FANG Zheng1, SUN Qiang1
收稿日期：2010-06-25 年卷（期）页码：2010,28(03):234-234-236
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：涎腺腺样囊性癌,苦参碱,人端粒酶催化亚基,细胞周期,
Key words：salivary adenoid cystic carcinoma,Matrine,human telomerase reverse transcriptase,cell cycle,
基金项目：
河南省教育厅自然科学基金资助项目（2008A320035）
中文摘要
目的观察中药苦参有效成分苦参碱对涎腺腺样囊性癌ACC-M细胞的细胞周期及端粒酶催化亚基（hTERT）表达的影响。方法体外培养ACC-M细胞，用不同浓度的苦参碱对体外培养的ACC-M细胞进行干预，以流式细胞仪检测对照组和不同浓度（0.25、0.50、0.75、1.00 mg·mL-1）苦参碱作用不同时间后细胞周期的变化；逆转录聚合酶链反应（RT-PCR）检测苦参碱作用48 h后细胞hTERT mRNA的表达改变；流式细胞术定量检测hTERT蛋白的表达变化。结果苦参碱作用后明显抑制ACC-M细胞的增殖，使ACC-M细胞周期阻滞于G0/G1期，并与药物浓度和作用时间呈正相关；苦参碱作用后hTERT基因和蛋白表达明显下降。结论苦参碱对ACC-M细胞有明显细胞周期阻滞作用，同时显著下调hTERT基因表达。细胞周期阻滞作用可能与苦参碱下调hTERT基因表达有关。
英文摘要
Objective To investigate the effect of the traditional Chinese medicine Matrine on cell cycle and human telomerase reverse transcriptase（hTERT） of human ACC-M cell lines. Methods Different concentrations of Matrine were used in the medium of ACC-M cells. Change of cell cycle were detected by flow cytometry after ACC-M cell were cultivated with different concentrations Matrine（0.25, 0.50, 0.75, 1.00 mg·mL-1）. Expression of hTERT was investigated by reverse transcription-polymerase chain raction（RT-PCR） and indirect immunofluorescene and flow cytometry quantitative analysis. Results Matrine caused obviously the G0/G1 phase block and inhibited proliferation of ACC-M cells. At same time, this effect was positive correlation to Matrine concentration and treat time. Matrine can inhibit the expression of hTERT mRNA and protein. Conclusion Matrine can obviously inhibit cell cycle and down-regulate expression of hTERT. Inhibition of cell cycle is possible correlation with down-regulation expression of hTERT.
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