牙周可疑致病菌代谢组学鉴定的初步研究
Preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method
作者：鲁维希 吴亚菲 肖丽英 李明云 郭强 熊萍 贾向明 肖晓蓉 朱硃 龚其美 李伟
Author：LU Wei-xi1, WU Ya-fei1, XIAO Li-ying1, LI Ming-yun1, GUO Qiang1, XIONG Ping1, JIA Xiang-ming2, XIAO Xiaorong1, ZHU Zhu1, GONG Qi-mei1, LI Wei1
收稿日期：2009-06-25 年卷（期）页码：2009,27(03):310-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：代谢组学,核磁共振,细菌,鉴定,
Key words：metabonomics,nuclear magnetic resonance,bacterium,identification,
基金项目：
教育部科学技术研究重大资助项目（307022）；四川省应用基础研究资助项目（07JY029-072）
中文摘要
目的分析比较牙周可疑致病菌的核磁共振代谢图谱，探讨用代谢组学方法快速鉴定口腔细菌的可能性。方法分别在BHI液体培养基中接种相同密度的牙龈卟啉单胞菌、中间普氏菌和具核梭杆菌，采用比浊法绘制生长曲线。取3种细菌生长稳定期的培养液进行核磁共振氢谱（1H-NMR）测定，用主成分分析法进行数据分析。结果主成分分析显示3组数据各自有较为集中的类聚关系，可以区分这3种细菌。结论代谢组学是一种具有良好应用前景的口腔细菌分类鉴定方法。
英文摘要
Objective To evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms. Methods Suspensions of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum with same density were prepared and cultured respectively at liquid BHI medium. Then the growth quantity was measured periodically through turbidimetry and the growth curves of the inoculated bacteria were completed. The culture solutions of stable growth phase were sampled and characterized by 1H-nuclear magnetic resonance（1H-NMR）. The data of 1HNMR spectroscope results were analyzed by principal components analysis（PCA）. Results The PCA showed the obvious clustering phenomena and the points of three groups differentially centralized to three clusters. Therefore, the NMRbased metabonomics profiles could discriminate the three different kinds of bacteria. Conclusion The metabonomics is a potential classable method to identify the oral pathogenic bacteria.
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