期刊导航

论文摘要

骨碎补柚皮苷对人牙周膜细胞增殖、碱性磷酸酶活性影响的实验研究

Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells

作者:蒋俊强 丁一 李小玉 蔡炜 王忠朝

Author:JIANG Jun -qiang1, DING Yi2, LI Xiao -yu3, CAI Wei1, WANG Zhong -chao1

收稿日期:2009-10-25          年卷(期)页码:2009,27(05):538-538-541

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:骨碎补柚皮苷,牙周膜细胞,增殖,碱性磷酸酶活性,

Key words:Drynaria fortunei naringin,periodontal ligament cells,proliferation,alkaline phosphatase activity,

基金项目:

国家自然科学基金资助项目(30640077)

中文摘要

目的研究骨碎补柚皮苷对人牙周膜细胞(hPDLC)增殖、碱性磷酸酶(ALP)活性的影响。方法采用酶消化结合组织块法,体外培养人牙周膜细胞;运用MTT法测定不同质量浓度骨碎补柚皮苷在不同时间对hPDLC增殖的影响;采用IFCC推荐法测定不同质量浓度骨碎补柚皮苷作用后hPDLC的ALP活性,采用考马氏亮兰法,测定样本中蛋白量,从而测定每毫克冻融蛋白中所含的ALP比活性。结果10、1、0.1 mg·L-1这3种质量浓度的骨碎补柚皮苷对hPDLC的增殖有促进作用;100、10、1、0.1 mg·L-1的骨碎补柚皮苷均可促进hPDLC的ALP活性,其中以1 mg·L-1组的促进作用最强。结论骨碎补柚皮苷对hPDLC具有促进增殖、促进碱性磷酸酶活性的作用。

英文摘要

Objective To evaluate the biological effects of Drynaria fortunei naringin on the proliferation and alkaline phosphatase(ALP) activity of human periodontal ligament cells(hPDLC). Methods hPDLC were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. 3-(4, 5-Dimethylthiazol-2- yl)-2, 5-diphenyltetrazolium bromide(MTT) method was used to observe the proliferation of hPDLC with the effects of different concentrations of Drynaria fortunei naringin at difference times. The method recommended by International Federation of Clinical Chemistry and Laboratory Medicine(IFCC) was adopted to investigate the effect of Drynaria fortune naringin on the alkaline phosphatase(ALP) activity of hPDLC. At the same time, we used bright blue method to detect the contents of protein in each sample. Then ALP activity in per milligram protein was accounted. Results Significant proliferative promotion to hPDLC by Drynaria fortunei naringin could be observed at the dose of 10, 1, 0.1 mg·L-1. Significant ALP activity of hPDLC promotion of Drynaria fortune naringin could be observed at the dose of 100, 10, 1, 0.1 mg·L-1 And the dose of 1 mg·L-1 Drynaria fortune naringin had greatest promotion on ALP activity of hPDLC. Conclusion Drynaria fortune naringin might significantly promote the proliferation and increase the ALP activity of hPDLC.

关闭

Copyright © 2020四川大学期刊社 版权所有.

地址:成都市一环路南一段24号

邮编:610065