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论文摘要

生存素小干扰RNA对人腺样囊性癌ACC-2细胞移植瘤体生长的抑制作用

Survivin small interfering RNA suppresses the growth of adenoid cystic carcinoma cell xenografts in vivo

作者:杨军 汪欣 许波 熊宇

Author:YANG Jun, WANG Xin, XU Bo, XIONG Yu

收稿日期:2009-08-25          年卷(期)页码:2009,27(04):433-

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:RNA干扰,小发夹结构RNA,腺样囊性癌,

Key words:RNA interference,short hairpin RNA,adenoid cystic carcinoma,

基金项目:

重庆市自然科学基金资助项目(CSTC,2007BB5064)

中文摘要

目的观察生存素(Survivin)小干扰RNA(siRNA)在体内是否能抑制腺样囊性癌移植瘤体的生长。方法裸鼠体内注射稳定转染靶向Survivin基因的shRNA真核表达载体pGenesil-shRNA-Survivin后的人腺样囊性癌细胞株ACC-2,观察移植肿瘤生长情况,以空白对照组及阴性质粒对照组作为对照。处死裸鼠后测量移植瘤体积;采用苏木精-伊红染色病理证实移植瘤后,免疫组织化学法检测移植瘤的Survivin蛋白表达情况,半定量RT-PCR检测Survivin mRNA表达情况。结果处死裸鼠后测量移植瘤体积表明,pGenesil-shRNA-Survivin组移植瘤体积明显减小;其Survivin mRNA及蛋白表达明显降低(P

英文摘要

Objective To study the effect of stable transfection of small hairpin RNA(shRNA) targeting Survivin gene on the growth of adenoid cystic carcinoma(ACC) xenografts in nude mice. Methods Adenoid cystic carcinoma cell-2 line(ACC-2) was transfected with Survivin targeting shRNA eukaryotic expression vector, and stable monoclonal cells were selected. Transfected and nontransfected cells were respectively inoculated into subcutaneous tissue of nude mice as experimental and controls. Survivin mRNA expression of transfected and nontransfected cells were detected by reverse transcription -polymerse chain reaction(RT -PCR). Survivin protein expression of tumors was investigated by immunohistochemistry. The growth of xenograft tumors was observed by measurement of tumor volume. Results Compared to controls, the expression of tumor volume decreased in transfected group by pGenesil-shRNA-Survivin. The expression of Survivin mRNA and protein was obviously reduced. Conclusion Survivin siRNA markedly suppressed the growth of adenoid cystic carcinoma cell xenografts in nude mice through lastingly inhibition effect on Survivin.

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