期刊导航

论文摘要

碱性成纤维细胞生长因子对牙周膜细胞表皮生长因子受体基因表达的影响

Effect of basic fibroblast growth factor on the gene expression of epidermal growth factor receptor by periodontal ligament cells in culture

作者:陈莹 林崇韬 高颖

Author:CHEN Ying, LIN Chong-tao, GAO Ying

收稿日期:2009-06-25          年卷(期)页码:2009,27(03):340-

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:碱性成纤维细胞生长因子,表皮生长因子受体,牙周膜细胞,细胞分化,

Key words:basic fibroblast growth factor,epidermal growth factor receptor,periodontal ligament cells,cell differentiation,

基金项目:

吉林省科技厅基金资助项目(200705342)

中文摘要

目的研究碱性成纤维细胞生长因子(bFGF)对人牙周膜细胞(PDLC)表达表皮生长因子受体(EGFR)的影响,探讨bFGF在牙周组织分化再生中的意义。方法体外原代培养人PDLC,有限稀释法形成单细胞克隆,用外源性bFGF刺激单细胞克隆,采用逆转录聚合酶链反应(RT-PCR)检测克隆细胞内EGFR基因表达的变化。结果bFGF促进人PDLC内EGFR mRNA的合成,并且随着质量浓度的增加促进作用增强。结论bFGF对EGFR的促进作用很可能是牙周炎损伤修复过程中一个重要的调节因素,为牙周组织分化再生提供部分理论基础。

英文摘要

Objective To study the effect of basic fibroblast growth factor(bFGF) on the gene expression of epidermal growth factor receptor(EGFR) by periodontal ligament cells(PDLC) in culture and discuss the effect of bFGF in cell differentiation and periodontal regeneration. Methods Human PDLC were cultured in vitro and cell clone was obtained by the method of limiting dilution. PDLC were stimulated by bFGF, and then gene expression of EGFR was assessed by reverse transcription-polymerase chain reaction(RT-PCR). Results The mRNA expression of EGFR was promoted by bFGF and the effect was dose-dependent. Conclusion The promotion effect of the mRNA expression of EGFR in PDLC may be one of important factors which participate in the healing process of periodontitis and provide partly theoretical basis of cell differentiation and periodontal regeneration.

关闭

Copyright © 2020四川大学期刊社 版权所有.

地址:成都市一环路南一段24号

邮编:610065