维生素E琥珀酸酯诱导舌鳞癌Tca8113细胞凋亡的研究
The study of apoptosis induction effect of vitamin E succinate on Tca8113 human tongue cancer cells
作者：曹选平 王树斌 周弘 吴豪阳 张彦喜 刘学军 张松涛
Author：CAO Xuan- ping1, WANG Shu- bin1, ZHOU Hong1, WU Hao- yang1, ZHANG Yan- xi1, LIU Xue- jun1, ZHANG Song- tao2
收稿日期：2008-08-25 年卷（期）页码：2008,26(04):362-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：维生素E琥珀酸酯,Tca8113细胞,凋亡,Fas蛋白,
Key words：vitamin E succinate,Tca8113 cell,apoptosis,Fas protein,
基金项目：
河南省科技创新人才工程基金资助项目（2002109）
中文摘要
目的探讨维生素E琥珀酸酯（VES）对人舌鳞癌Tca8113细胞的凋亡诱导作用及其可能的分子机制。方法四甲基偶氮唑蓝（MTT）检测VES对舌鳞癌Tca8113细胞的抑制作用；应用流式细胞仪分析不同质量浓度VES处理后舌鳞癌细胞的凋亡率；Fas中和抗体进行阻断实验；以细胞免疫化学法和流式细胞仪检测VES处理后肿瘤细胞Fas蛋白水平和细胞表面Fas表达的变化。结果不同质量浓度的VES处理后，舌鳞癌细胞的生长受到明显的抑制，肿瘤细胞的凋亡率显著上升，呈现时间依赖性和剂量依赖性；Fas中和抗体进行阻断后，细胞凋亡率显著下降；VES处理后细胞Fas蛋白表达增强；流式细胞仪检测细胞表面Fas平均荧光强度也显著升高。结论VES对舌鳞癌细胞具有凋亡诱导作用，其作用机制与肿瘤细胞表面Fas蛋白表达的上调有关。
英文摘要
Objective To investigate the apoptosis induction effect of vitamin E succinate（VES） on Tca8113 cells and its possible mechanisms. Methods The proliferative activity of Tca8113 was assessed by methyl thiazolyl tetrazolium（MTT） assay. After Tca8113 cells were treated with different concentrations of VES, apoptotic rates were analyzed by flow cytometry（FCM）. Fas monoclonal antibody was used for the blocking test. Fas expression was detected by immuocytochemistry（SABC assay） and FCM. Results VES demonstrated a significant growth inhibitory effect and apoptosis induced effect on the Tca8113 cells in a dose- and time- dependent manner. Fas neutralizing antibody can block the apoptosis induced by VES. After the administration of VES, the expression of Fas protein increased and the kytoplasm staining enhanced. Proteinum quantitative analysis showed that the mean fluorescence intensity increased. Conclusion VES can induce apoptosis in human tongue cancer cells, and the up- regulation of the cell surface Fas protein may play an important role in the process.
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