口腔癌相关成纤维细胞对舌癌细胞株细胞外信号调节激酶通路的影响
Effect of or al car cinoma- associated fibroblasts on extr acellular signal- r egulated kinases pathway in the lingual car cinoma cell line
作者：林靖雯 陈谦明 李胜富 付春华 林梅 龙丹 周红梅
Author：LIN Jing- wen1, CHEN Qian- ming2, LI Sheng- fu3, FU Chun- hua1, LIN Mei2, LONG Dan3, ZHOU Hong- mei2
收稿日期：2007-10-25 年卷（期）页码：2007,25(05):490-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：癌相关成纤维细胞,舌癌,细胞外信号调节激酶传导通路,
Key words：carcinoma- associated fibroblasts,tongue carcinoma,extracellular signal- regulated kinases pathway,
基金项目：
国家自然科学基金资助项目（30572035）；四川省科技攻关资助项目（05SG022- 018- 1）
中文摘要
目的研究口腔癌相关成纤维细胞（CAFs）对舌癌细胞株细胞外信号调节激酶（ERK）通路的影响。方法以口腔CAFs条件培养基刺激舌癌细胞株Tca8113和将Tca8113细胞与口腔CAFs共同培养，采用Western杂交检测特定时间Tca8113细胞总ERK和总pERK的表达。结果Tca8113细胞经口腔CAFs条件培养基刺激或与口腔CAFs共同培养后，总pERK的表达迅速增加，总pERK与总ERK的比值增加。结论口腔CAFs对癌细胞ERK通路有活化作用。
英文摘要
Objective To investigate the effects of oral carcinoma - associated fibroblasts（CAFs） on extracellular signal- regulated kinases（ERK） pathway in a lingual carcinoma cell line. Methods The lingual carcinoma cell line. Tca8113 was stimulated by conditioned medium from oral CAFs, or cocultured with oral CAFs for definite time. Total ERK and pERK in Tca8113 lysate were detected by Western blotting, and the ratio between pERK and ERK were calculated. Results Both stimulation by conditioned medium and coculture induced prompt phosphorylation of ERK, and increased the ratio between pERK and ERK. Conclusion Oral CAFs can activate ERK pathway of carcinoma cells.
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