人脱细胞真皮基质的结构及与MG63成骨样细胞生物相容性的研究

Objective To study the microstructue of acellular dermal matrix（ADM） and the attachment and proliferation of MG63 osteoblast - like cells on the membrane. Methods ADM was experimental group and expanded polytetrafluoroethylene（e- PTFE） membrane was control group. Light microscope and scanning electronic microscope （SEM）were used to observe the microstructure of the two kinds of membrane. MG63 osteoblast- like cells were cultured on the two kinds of membrane and the blank group. The cell viability was checked by cell viability analyzer （CVA） and alkaline phosphatase（ALP） activity was detected by ELISA. SEM was used to observe the adhesion and proliferation of the MG63 osteoblast- like cells on the two kinds of membrane. Results The ADM was composed of the tissular side and basal lamina side. The tissue side showed scale- shaped structure and the basal lamina side demonstrated many digitations and some pores of folliculus pili. The e- PTFE showed many tiny lined cracks in elliptic structure. The viability of the MG63 osteoblast- like cells on the two kinds of membranes and ALP measurement showed that there was no significant difference between each of the two membrane groups and the blank. The adhesion and proliferation of osteoblast- like cells on the two kinks of membranes were both well. Conclusion ADM has no negative effects on the growth of osteoblast - like cells. ADM is biocompatibile and its microstructure is appropriate for guided bone regeneration materials.