盐酸小檗碱对大鼠牙周组织中白细胞介素-1β和肿瘤坏死因子-α表达的影响
Effect of berber ine hydrochlor ide on the expr ession of inter leukin- 1β and tumor necrosis factor - α in periodontal tissues in r ats
作者：余占海 张国英 张小恒 杜建东 杨倩 何福德
Author：YU Zhan- hai, ZHANG Guo- ying，ZHANG Xiao- heng, DU Jian- dong, YANG Qian, HE Fu- de
收稿日期：2008-02-25 年卷（期）页码：2008,26(01):82-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：盐酸小檗碱,牙周炎模型,白细胞介素- 1β,肿瘤坏死因子- α,
Key words：berberinehydrochloride;berberine hydrochloride,periodontitis model,interleukin- 1β,tumor necrosis factor- α,
基金项目：
甘肃省科技攻关基金资助项目（2GS064- A43- 020- 34）；兰州市科技攻关基金资助项目（06- 1- 1）
中文摘要
目的探讨盐酸小檗碱对大鼠实验性牙周炎模型牙周组织中白细胞介素- 1β（IL- 1β）和肿瘤坏死因子- α（TNF- α）表达的影响。方法在成功建立大鼠实验性牙周炎模型的基础上，实验动物在分别灌服盐酸小檗碱后第1、2、3、4周处死，取牙周组织标本作切片，行常规苏木精- 伊红染色，观察牙周组织病理变化状况，并采用免疫组织化学SABC法检测IL- 1β、TNF- α水平变化。结果牙周炎组牙周组织中IL- 1β、TNF- α表达明显高于正常组（P
英文摘要
Objective To determine the effects of berberine hydrochloride on the expressions of interleukin- 1β （IL- 1β） and tumor necrosis factor- α（TNF- α） in periodontal tissues in rat periodontitis model. Methods Based on the successful rat periodontitis model, the experimental rats were randomized into different groups followed by oral treatment with berberine hydrochloride for 1, 2, 3, 4 weeks and then the rats were sacrificed and analyzed. Pathological assay and HE staining were used to detect the general conditions and pathological changes of rat periodontal tissues. And immunohistochemical staining was conducted to determine the expressions of IL- 1β and TNF- α in rats periodontitis model periodontal tissues. Results The levels of IL- 1β and TNF- α in the periodontitis tissues were significantly higher than that in the control group. Treatment with berberine hydrochloride decreased the levels of IL- 1βand TNF- αin periodontitis tissues（P
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