Objective To investigate the effects of time varied stress on the proliferation of myoblast in rats and provide the basic experimental data for the remodeling of tissue in functional orthopaedics. Methods Based on the pulsatile mechanical system founded, this study loaded different strain(2.5, 5.0, 10.0 kPa) to the myoblast of lateral pterygoid muscle. The proliferation of myoblast was detected by 3H- TDR. Results After 6 hours under time varied strain, the significant proliferation of myoblast(P