Objective To provide content- measuring evidences for establishing the quality criteria of dexamethasone acetate ulcer pasta. Methods Content of dexamethasone acetate was assayed by the isonicotinyl hydrazine(INH) colorimetry. Results The good linear range was 6 - 16 μg/mL(r=0.999 1). The average recovery of dexamethasone acetate was 98.9%, and relative standard deviation was low than 1%(n=5). Conclusion It is easy, convenient, accurate and dependable for INH colorimetry to determine the content of dexamethasone acetate.