Objective To evaluate the effect of non- surgical treatment with Florida probe. Methods 100 patients with periodontitis were chosen in the study, who accepted periodontal non- surgical treatment. Pocket depth(PD) and attachment loss(AL) of all patients were recorded with Florida probe before and at the first month after periodontal non- surgical treatment. The detecting sites were mesialbuccal, buccal, distalbuccal and lingual. All teeth were divided into four groups: Anterior teeth group, premolar group, molar group and all teeth group. The therapeutic efficacy of PD and AL of groups, sites and different pocket depths was compared. Results In all four groups, PD, AL before and after one month periodontal non- surgical treatment demonstrated significant differences(P0.05). Sites had no significant difference(P>0.05). Conclusion Florida probe evaluates periodontal conditions accurately and objectively. Periodontal non- surgical treatment allowed for favourable treatment response on periodontitis, anterior teeth had a good therapeutic efficacy, different sites had the equal therapeutic efficacy.