人牙髓细胞与牙龈成纤维细胞差异表达基因的克隆及特征分析
Cloning and Char acter ization of Genes Differ entially Expr essed in Human Dental Pulp Cells and Gingival Fibroblasts
作者：王忠东;吴纪楠;周琳;凌均棨;郭希民;肖明振;朱峰;蒲勤;柴玉波;赵忠良
Author：WANG Zhong- dong1, WU Ji- nan2, ZHOU Lin2, LING Jun- qi1, GUO Xi- min3, XIAO Ming- zhen4, ZHU Feng5, PU Qin5, CHAI Yu- bo5, ZHAO Zhong- liang5
收稿日期：2007-02-25 年卷（期）页码：2007,25(01):75-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙髓细胞,牙龈成纤维细胞,差异表达,消减文库,
Key words：dental pulp cell,gingival fibroblast,differential expression,subtractive hybridization,
基金项目：
国家自然科学基金资助项目（30471889）
中文摘要
目的批量克隆人牙髓细胞（HDPC）与人牙龈成纤维细胞（HGF）的差异表达基因并对其特征进行分析，研究HDPC的生物学特性。方法体外培养HDPC和HGF，应用基于PCR的改良消减杂交技术构建HDPC和HGF的cDNA消减文库，批量克隆HDPC和HGF的差异表达基因并测序，使用GenBank的BLAST对测序结果进行同源序列比较。结果经过序列测定，获得12个差异表达基因的序列，经BLAST分析有2个为未知基因。在已知基因中，含有4个与细胞信号转导机制相关的基因；2个与细胞转运机制相关的基因（包括细胞膜及细胞核膜转运）；2个与细胞RNA剪接机制相关的基因。结论HDPC的生物学特性是由某些特定基因的差异表达所决定的，其生长、分化机制可能与相对旺盛的蛋白合成及分泌活性相关。
英文摘要
Objective To study the biological properties of human dental pulp cells（HDPC）by cloning and analysis of genes differentially expressed in HDPC in comparison with human gingival fibroblasts（HGF）. Methods HDPC and HGF were cultured and identified by immunocytochemistry. HPDC and HGF subtractive cDNA library was estab - lished by PCR- based modified subtractive hybridization, genes differentially expressed by HPDC were cloned, se - quenced and compared to find homogeneous sequence in GenBank by BLAST. Results Cloning and sequencing analysis indicate 12 genes differentially expressed were obtained, in which two were unknown genes. Among the 10 known genes, 4 were related to signal transduction, 2 were related to trans-membrane transportation（both cell membrane and nuclear membrane）, and 2 were related to RNA splicing mechanisms. Conclusion The biological properties of HPDC are determined by the differential expression of some genes and the growth and differentiation of HPDC are associated to the dynamic protein synthesis and secretion activities of the cell.
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