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论文摘要

实时定量PCR分析脂肪酸合酶在口腔鳞癌中的表达

Analysis of Gene Expression of Fatty Acid Synthase in Squamous Cell Carcinoma of the Oral Cavity by the Method of Real-time Quantitative PCR

作者:王玉凤;王晓毅;高庆红;杨晓勇;王洪萍;曾磊

Author:WA NG Yu-fe,WA NG Xiao-yi,GA 0 Qing-honf,YA NG Xiao-yong2,WANG Hong-ping2,ZENG Lei2

收稿日期:2006-12-25          年卷(期)页码:2006,24(06):551-

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:口腔鳞状细胞癌,实时定量PCR,脂肪酸合酶,

Key words:squamous cell carcinoma of oral cavity,real-time quantitative PCR,fatty acid synthase,

基金项目:

国家自然科学基金资助项目(30550002)

中文摘要

目的利用实时定量PCR(RT—PCR)相对定量脂肪酸合酶(FAS)在口腔鳞癌、癌周以及正常口腔组织中的表达情况。方法切取口腔鳞癌患者新鲜手术标本的癌组织、癌周组织及正常组织,将组织切剪成碎块,提取细胞总RNA,经寡聚脱氧胸腺嘧啶逆转录,实时定量PCR扩增,针对内参照GAPDH基因相对定量FAS的表达,比较FAS在3种组织中的表达。结果 FAS在癌组织中高表达,19例患者癌组织中的FAS mRNA表达水平皆高于正常组织,最高达16倍Go

英文摘要

Objective To relatively quanti~ the gene expression of fatty acid synthase in squamous cell carcinoma, adjacent tissue,and some normal oral tissues by real-time quantitative PCR.Methods The tissues were collected flesh from surgical specimens.The collected tissues were minced.Then the total RNA was extracted. The RNA was reversely transcripted into cDNA with random prime.And then the cDNA was amplified by real-time quantitative PCR to quantify the gene expression of FAS according to an internal control GAPDH.The diference of FAS gene expression Was compared between squamous cell carcinoma,adjacent tissue,and some normal oral tissues.Results The expression of FAS of squamous cell carcinoma Was notably higher than the other two(P

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