铸造合金全冠戴用后颊黏膜上皮细胞DNA损伤的研究
Study of DNA Damage of Buccal Mucosal Cells after Wearing Casting Alloy Crowns
作者：苏俭生;邓章悦;邵磊;乔广艳;万书健
Author：SU Jian-sheng1, DENG Zhang-yue1, SHAO Lei2, QIAO Guang-yan1, WAN Shu-jian1
收稿日期：2006-02-25 年卷（期）页码：2006,24(01):21-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙科铸造合金,离子析出,颊黏膜上皮,细胞DNA,
Key words：dentalcastingalloy,ionreleasing,buccalmucosalcells,DNA,
基金项目：
上海市科委科技攻关重点资助项目（034119839）
中文摘要
目的 研究3种铸造合金全冠戴用后颊黏膜上皮细胞DNA的损伤情况。方法 给狗分别进行58%金合金、不含Be元素的NiCr合金、含Be元素的NiCr合金铸造全冠修复，建立实验动物模型。采用VG-X7型电感耦合等离子体质谱仪和IRIS Intrepid型电感耦合等离子体原子发射光谱仪检测戴冠前、戴冠2周、1月、2月及3月时颊黏膜上皮细胞中金属离子的种类和质量浓度，同时应用彗星电泳方法检测颊黏膜上皮细胞DNA的损伤情况。结果 58%金合金全冠戴用后，在颊黏膜上皮细胞中金属离子质量浓度变化不明显；戴冠2周时彗星数目显著增加，而后又恢复到戴冠前水平。NiCr合金全冠戴用后，颊黏膜上皮细胞中Ni、Cr、Be离子质量浓度以及彗星数目均随着戴冠时间的延长而逐渐升高，其中含Be元素组的彗星数目显著高于不含Be元素组，而后者彗星数目又明显高于58%金合金组。颊黏膜上皮细胞中的Ni、Cr、Be离子质量浓度与彗星数目呈正相关。结论 金合金全冠戴用后没有引起颊黏膜上皮细胞DNA的损伤，而NiCr合金全冠，尤其含Be元素者，戴用后将引起颊黏膜上皮细胞DNA发生明显损伤。
英文摘要
Objective To test the corrosion behavior of three kinds of dental casting alloys and to investigate the effect of the released metal ions on the DNA damage of dog buccal mucosal cells. Methods Three kinds of frequently used dental casting alloys were used to make full crowns for dogs. The concentration of the released metal ions was measured after the restoration of 2 weeks, 1 month, 2 months and 3 months. The DNA damage of buccal mucosal cells was studied by the method of SCGE. Results The metal ions released from NiCr and NiCrBe were detected in buccal mucosal cells while the amount of the ions released from noble alloy（gold 58%）was too small to be detected. The DNA damage of mucosal cells increased after restoration of NiCr and NiCrBe crowns. Conclusion The noble alloy（gold 58%）is most corrosion resistant of the three alloys and has good biocompatibility. The NiCr and NiCrBe are prone to corrode and have cytotoxicity to cells.
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