表皮生长因子受体在人牙周膜成纤维细胞体外矿化中的作用
Expression of Epidermal Growth Factor Receptor in Human Periodontal Ligament Cells during Their Mineralization in vitro
作者：李纾;杨丕山;曹金芳;葛少华;潘克清
Author：LI Shu1，YANG Pi-shan1, CAO Jin-fang2, GE Shao-hua1, PAN Ke-qing1
收稿日期：2006-02-25 年卷（期）页码：2006,24(01):11-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：表皮生长因子受体,牙周膜成纤维细胞,矿化,细胞培养,
Key words：epidermalgrowthfactorreceptor,periodontalligamentcells,mineralization,cellculture,
基金项目：
山东省中青年科学家奖励基金资助项目（02BS096）
中文摘要
目的 探讨表皮生长因子受体（EGFR）在人牙周膜成纤维细胞（hPDLC）体外矿化中的作用。方法 体外培养hPDLC，运用免疫细胞化学技术，对hPDLC体外矿化前后EGFR的表达进行比较研究；应用原位杂交及RT-PCR技术，对矿化前后的hPDLC mRNA进行检测。结果 诱导前EGFR呈高表达，随着地塞米松诱导时间的延长，其表达逐渐下降，在矿化结节形成后的第4周，几乎呈阴性表达，与成骨细胞中EGFR的表达一致。原位杂交中杂交信号与免疫细胞化学的检测结果基本一致，RT-PCR的mRNA半定量检测发现，随着hPDLC的体外矿化，碱性磷酸酶升高，EGFR表达降低。结论 EGFR在hPDLC的矿化过程中起着负调控作用，与牙周膜自身结构的稳定性有关。
英文摘要
Objective To investigate the expression of epidermal growth factor receptor（EGFR） during the mineralization of human periodontal ligament cells（hPDLC）in vitro. Methods Studies using specific antibodies to immunolocalize EGFR in the mineral differentiating hPDLC were undertaken to investigate the different expression during the inducing process. In situ hybridization and RT-PCR technique were used to investigate the transcripts encoding the protein of EGFR. Results The results showed that immunocytochemical labeling gradually decreased following the elong of the induce time, downing to nearly negative at the 4th week and the signal of EGFR transcripts was weaker in the induced hPDLC than that in uninduced. Conclusion EGFR has a negative regulation function during the mineralization of hPDLC.
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