涎腺腺样囊性癌p16基因失活机制的研究
Mechanism of p16 Gene Inactivation in Salivary Adenoid Cystic Carcinoma
作者：郭小玲,孙善珍,魏奉才
Author：GUOXiao-ling1,SUNShan-zhen1,WEIFeng- cai2
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):418-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：涎腺腺样囊性癌,p16,缺失,突变,甲基化,
Key words：salivary gland adenoid cystic carcinoma,p16,deletion,mutation,methylation,
基金项目：山东省卫生厅基金资助项目(981010108)
中文摘要
目的 研究p16基因在涎腺腺样囊性癌中的失活机制。方法 收集山东大学齐鲁医院及口腔医院口腔
颌面外科涎腺腺样囊性癌新鲜标本53例,应用PCR、聚合酶链反应-单链构象多态性分析(PCR-SSCP)及甲基化特
异性PCR(MSP)技术检测涎腺腺样囊性癌中p16基因的纯合性缺失、突变及甲基化。结果 53个病例中16例
(30·2%)纯合性缺失,4例(7·5%)突变,26例(49·1%)高甲基化。结论 p16基因在涎腺腺样囊性癌中的主要失活
机制是启动子高甲基化和纯合性缺失,而基因突变比较少见。
英文摘要
Objective To study the mechanism of p16 gene inactivation in salivary adenoid cystic carcinoma.Methods 53 cases of freshly excised salivary adenoid cystic carcinomas were studied. Polymerase chain reaction (PCR) and single-stranded conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) were used to detect deletion and mutation of p16 gene in salivary adenoid cystic carcinomas. Methylation specific PCR (MSP) was used to detect the methylation status of p16 gene.Results The homozygous deletion, mutation and hypermethylation of p16 gene were noted in 16 cases (30·2%),4 cases (7·5%) and 26 cases (49·1%) respectively in 53 cases of salivary adenoid cystic carcinomas.Conclusion The main in- activation mechanisms of p16 gene in salivary adenoid cystic carcinomawere hypermethylation and homozygous deletion. The muta- tion p16 gene was rare in salivary adenoid cystic carcinoma.
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