成骨细胞中激活剂蛋白-1家族成员对流体剪切力的响应
ctivating Protein-1 Members in Response to Changes of Wall-shear Stress in Osteoblastic Cells
作者：朱赴东,赵士芳
Author：ZHUFu-dong,ZHAO Shi-fang
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):380-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：流体剪切力,成骨细胞,激活剂蛋白-1,
Key words：wall-shear stress,osteoblastic cells,activating protein-1,
基金项目：
浙江省自然科学基金资助项目(491060-N20078)
中文摘要
目的 研究激活剂蛋白-1家族成员FosB,c-Fos, c-Jun,JunD,JunB ,Fra-1和Fra-2对不同大小的流体剪切力
的生理响应。方法 对新生SD大鼠颅盖骨中分离出的成骨细胞施加流体剪切力,分成4组,每组加载的水平剪切
力大小分别为0·8 Pa,1·2 Pa,1·4 Pa及1·6 Pa。每组在加载剪切力0 min,10 min,15 min,30 min,60 min后分别用逆
转录聚合酶链反应测试FosB,c-Fos,c-Jun,JunD,JunB ,Fra-1和Fra-2 mRNA的表达。结果 FosB,c-Fos,c-Jun,JunD和
JunB在水平剪切力加载15 min时表达明显增高(P0·05)。当剪切力为1·2 Pa时,FosB,c-Fos,c-Jun,JunD和JunB mRNA表达明显高
于其他各组和空白对照组(P
英文摘要
Objective To observe activating protein-1(AP-1) members in response to changes ofwall-shear stress in osteoblas- tic cellsin vitro.Methods Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and sub- cultured. The third generation cellswere subjected towall-shear stress of 0.8 Pa,1.2 Pa,1.4 Pa and 1.6 Pa separately. Gene ex- pression of the seven AP-1 members were studied before (0 h) and 10 min,15 min,30 min,60 min after treated with wall-shear stress.Results The expression of FosB,c-Fos, c-Jun,JunD and JunB mRNA increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells compared to 0.8 Pa ,1.4 Pa and 1.6 Pa stress, and peaked at 15 min.Conclusion Me- chanical environment changes in osteoblastic cells induced a dramatic induction of most of the AP-1 members.
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