成骨细胞与血管内皮细胞联合培养对成骨细胞功能的影响
Influence of Vascular Cell Co-cultured with Osteoblastin vitroon Ability of Osteoblast
作者：张健,胡敏,张文怡
Author：ZHANGJian1, HUMin1, ZHANG Wen-yi2
收稿日期：2005-08-25 年卷（期）页码：2005,23(04):325-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：成骨细胞,血管内皮细胞,碱性磷酸酶,骨钙素,
Key words：osteoblast,vascular endothelial cell,alkaline phosphatase,osteocaclin,
基金项目：全军“十五”医药卫生科研基金面上课题资助项目
(01MA111)
中文摘要
目的 探讨不同比例兔成骨细胞(ROB)与血管内皮细胞(RVEC)直接联合共培养后,对成骨细胞功能的影
响。方法 取兔成骨细胞与血管内皮细胞,将两者分为4组分别以1∶0、1∶1、2∶1、4∶1直接联合共培养于培养皿中,
通过对细胞内碱性磷酸酶活性(ALP)测定和培养液中骨钙素(OC)定量测定,观察血管内皮细胞对成骨细胞功能的
影响。结果 成骨细胞与血管内皮细胞直接联合共培养相容性良好,2∶1的细胞比例组ALP活性及OC含量较其
他各组明显增高。结论 在体外直接联合共培养的体系中,血管内皮细胞有明显促进成骨细胞活性的作用。
英文摘要
Objective The purpose of this study was to observe the influence on proliferation of rabbit osteoblast (ROB) of rabbit vascular endothelial cell(RVEC) co-culturedwith rabbit osteoblast (ROB).Methods Rabbit osteoblasts and rabbit vascu- lar endothelial cells in ratio of 1∶0,1∶1,2∶1,4∶1were selected to co-culture.The function of osteoblasts was assessed by alkaline phosphatase(ALP) activity and osteocalcin(OC) assay.Results There was good compatibilitywhen osteoblasts and vascular en- dothelial cells were co-cultured. The activity ofALP and OC in the group of ROB and RVEC co-cultured in ratio of 2∶1 was higher than that of the other three groups.Conclusion In direct co-culture systemin vitro, RVEC can improve activity of ROB.
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