MAb225对Tca8113舌鳞癌细胞放射敏感性的影响
Effect of MAb225 on Radiosensitivity of Tca8113 Tongue Squamous Cell Carcinoma
作者：王明国,王中和,高萍,郜青,李迎,蔡以理
Author：WANGMing-guo1,WANGZhong-he2, GAOPing1, GAO Qing1, LI Ying1, CAI Yi-li2
收稿日期：2005-08-25 年卷（期）页码：2005,23(04):322-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：表皮生长因子受体,单克隆抗体,放射,鳞癌,
Key words：epidermal growth factor receptor,monoclonal antibody,radiosensitivity,squamous cell carcinoma,
基金项目：上海市口腔研究所基金资助项目(Y0215)
中文摘要
目的 探索表皮生长因子受体(EGFR)单克隆抗体MAb225对舌鳞癌细胞Tca8113的放射敏感性的调控作
用。方法 不同浓度的MAb225处理Tca8113舌鳞癌细胞,通过集落形成实验单靶多击模型拟合放射生存曲线,分
析细胞的增敏比(SERD0)和存活分数(SF2)的变化。同时,建立裸鼠移植瘤模型,单独及联合应用MAb225和放射处
理裸鼠移植瘤,通过生长延缓实验观察肿瘤生长变化,并进行统计学分析。结果 MAb225有明显的放射增敏作
用,0·5 mg/LMAb225作用后的SERD0为1·23,在一定的范围内,其增敏作用与剂量存在正相关;MAb225明显提高放
射对裸鼠移植瘤的杀伤作用(P
英文摘要
Objective The purpose of this study was to invest the effect of epidermal growth factor receptor monoclonal anti- body MAb225 on radiosensitivity of tongue squamous cell carcinoma cell Tca8113.Methods Tca8113 cells were treated with different concentrations of MAb225. Radiation dose survival curve was generated from clonogenic survival assay. SERD0and sur- vival faction (SF2) was analysed by single hit multi-target (SHMT) radiobiological model using RADMEDIC software. Nude mice with Tca8113 tumor xenografts were treated withMAb225, radiation treatment or both of them. Tumor responses were assessed by tumor growth delay, andttestwas used for statistical analysis.Results MAb225 enhanced the radiosensitivity ofTca8113 cells. SERD0of Tca8113 cells treated with 0·5 mg/LMAb225 was 1·23. The survival fraction of cells treated withMAb225 was signifi- cantly decreased. Tumor radioresponse could be enhanced by MAb225 (P
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