正畸移动后大鼠牙周炎牙整合素β1mRNA变化的实验研究
The Integrinβ1 mRNA Changes after Orthodontic Movement of Teeth in Periodontitis Rats
作者：张京剧,陈扬熙,肖立伟,段培佳,赵青
Author：ZHANG Jing-ju1, CHEN Yang-xi2, XIAO Li-wei2, DUANPei-jia2, ZHAO Qing2
收稿日期：2005-04-25 年卷（期）页码：2005,23(02):161-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：整合素β1,破骨细胞,正畸牙移动,牙周炎,
Key words：integrinβ1,osteoclast,orthodontic tooth movement,periodontitis,
基金项目：
教育部高等学校博士学科点专项科研基金资助项目 (20020610063)
中文摘要
目的 研究正畸移动后大鼠牙周炎牙与正常牙整合素β1 mRNA表达的变化。方法 选用10周龄雄性成
年SD大鼠96只,随机分为正常牙组、牙周炎牙组,每组48只。分别在加力后0、1、2、3、5、7 d点处死动物,制备标
本,采用原位杂交检测牙周炎牙与正常牙正畸移动后β1 mRNA转录水平的变化。结果 正常牙组和牙周炎组加力
后各时间点的整合素β1 mRNA阳性强度均较加力前有明显增高(P0·05)。结论 在牙移动中,整合素β1 mRNA牙周组织破骨细胞上有强表
达,提示其与牙移动中牙周组织的稳定和改建有关。
英文摘要
Objective To study the integrinβ1 mRNA changes after orthodontic tooth movement in normal teeth and periodon- titis teeth of rats.Methods The OD of positively stained osteoclasts for integrinβ1 mRNAusing in situ hybridzationwas detected after orthodontic tooth movement in normal teeth and periodontitis teeth groups.Results Integrinβ1 mRNA expression were de- tected on all osteoclasts in toothmovement samples of normal and periodontitis teeth. There were stronger positive signals after giv- en orthodontic force in both of the two groups. But no differenceswere found after 0·5, 1, 2, 3, 5, 7, 10 days since orthodontic tooth movement. The integrinβ1 mRNAsignals in normal toothmovement groupwere not different fromthat in periodontitis group. Conclusion The integrinβ1 of osteoclasts may play a role in the stability and remodeling of periodontal ligament in orthodontic tooth movement. There were no difference in the OD of integrinβ1 mRNA staining in orthodontic tooth movement between normal teeth group and periodontitis teeth group.
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