碱性成纤维细胞生长因子对成骨细胞和牙周膜成纤维细胞迁移、增殖的影响
Influence of Basic Fibroblast Growth Factors on the Migration, Proliferation of Osteoblasts and Periodontal Ligament Fi- broblasts
作者：谭震,宫苹,赵青
Author：TANZhen,GONG Ping,ZHAO Qing
收稿日期：2005-06-25 年卷（期）页码：2005,23(03):201-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙周膜成纤维细胞,成骨细胞,碱性成纤维细胞生长因子,
Key words：periodontal ligament fibroblasts,osteoblasts,basic fibroblast growth factors,
基金项目：
高等学校博士学科点专项科研基金资助项目 (B120010610059)
中文摘要
目的 明确碱性成纤维细胞生长因子(bFGF)对体外成骨细胞和牙周膜成纤维细胞迁移、增殖的影响,以
探讨在牙种植体组织界面局部应用bFGF诱导类牙周膜形成的可行性。方法 同一只SD大鼠来源的成骨细胞和
牙周膜成纤维细胞经传代培养至第4代,建立体外创伤模型,分别在普通培养基和含bFGF的培养基中培养,观察
细胞迁移情况,四唑盐比色实验(MTT)测定细胞的增殖速度。结果 普通培养基中,成骨细胞迁移速度快于成纤维
细胞。加bFGF培养基中牙周膜成纤维细胞迁移速度明显快于其他各组,同时MTT结果显示加入bFGF能明显促进
两种细胞的增殖。结论 bFGF能明显促进牙周膜成纤维细胞的增殖、移行。
英文摘要
Objective In order to determine the influence of bFGF on the biological characteristics of osteoblasts and periodon- tal ligament cells, the author investigate the influence of bFGF on the migration, proliferation of osteoblasts and periodontal liga- ment fibroblasts between osteoblasts and fibroblasts.Methods The forth generation of osteoblasts and periodontal ligament fibro- blast were obtained and the woundingmodelin vitroof themwas founded. The cellswere cultured in mediumswith orwithout bF- GF. Their migration and proliferation were observed and measured.Results Osteoblasts migrated faster than fibroblasts in com- mon culture mediumwithout bFGF and the periodontal ligament fibroblasts in culture mediumwith bFGF had higher migration rate than other groups. bFGF promoted the proliferation of both two kinds of cells.Conclusion bFGF could accelerate periodontal fi- broblasts′proliferation and migration.
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