新型多孔纳米双相磷酸钙陶瓷支架体外细胞相容性的实验研究
The Study of Cell Biocompatibility of New Pattern Biphasic Calcium Phosphate Nanocompositein vitro
作者：王涛,田卫东,刘磊,陈希哲,廖运茂,李声伟
Author：WANG Tao, TIANWei-dong, LIULei, CHENGXi-zhe, LIAO Yun-mao, LISheng-wei.
收稿日期：2005-04-25 年卷（期）页码：2005,23(02):106-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：骨髓基质细胞,纳米双相磷酸钙陶瓷支架,组织工程,
Key words：bone marrow stromal cells,porous biphasic calcium phosphate nanocomposite,tissue engineering,
基金项目：
四川省科技攻关资助项目(03GG009-027,03SG022-003-1); 高等学校优秀青年教师教学科研奖励计划资助项目(2003682)
中文摘要
目的 评价新型多孔纳米双相磷酸钙陶瓷支架材料的体外细胞相容性。方法 SD大鼠骨髓基质细胞 (BMSCs)经矿化诱导培养、扩增并检测证实其已具成骨细胞表型后,分别与多孔纳米双相磷酸钙陶瓷支架(实验组)、普通多孔羟基磷灰石陶瓷支架(对照组)体外复合培养。扫描电镜观察BMSCs在材料上的生长及生理功能表达情况;测定细胞碱性磷酸酶活性及骨钙素的含量;流式细胞仪检测细胞的凋亡及周期、倍体,比较细胞的粘附能力、增殖活力及成骨活性。结果 BMSCs经体外诱导形成钙结节,Ⅰ型胶原和碱性磷酸酶免疫染色结果阳性。两组材料上皆有细胞附着生长,但实验组细胞的粘附能力、增殖活力及成骨活性均强于对照组。结论 SD大鼠BM- SCs与新型多孔纳米双相磷酸钙陶瓷支架材料有良好的细胞相容性。
英文摘要
Objective To study the cell biocompatibility of porous biphasic calcium phosphate nanocompositein vitro.Meth- ods Bone marrowmesenchymal cell(BMSCs) obtained from SD rat bone marrow werein vitroinduced and proliferated. After theirosteoblast phenotypes were verified, BMSCs were seeded onto prepared porous biphasic calcium phosphate nanocomposite (Experiment group) and common porous hydroxyapatite (Control group). The cell adhesion was evaluated by scanning electron microscope. Synthesis of alkaline phosphatase enzyme (ALP) and osteocalcin were detected and cell cycle was detected by flow cytometry.Results BMSCs could fully attach to and extend on the material in experiment and control group, Moreover, experi- ment groupwere superiorto control group in adhesion, proliferative abilities and osteogenic activity.Conclusion BMSCs can dif- ferentiate to osteoblast phenotype; the porous biphasic calcium phosphate nanocomposite as bone tissue engineering scaffold has good cell biocompatibility.
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