碱性成纤维细胞生长因子对犬牙周成纤维细胞样细胞增殖影响的体内研究
The Effect of bFGF on Proliferation of Periodontal Fibroblast-like Cells of Dogs
作者：杨丕山,孙钦峰,宋爱梅,葛少华
Author：YANGPi-shan1,SUNQin-feng1,SONG Ai-mei2,GE Shao-hua1
收稿日期：2004-02-25 年卷（期）页码：2004,22(01):59-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：成纤维细胞生长因子,牙周再生,成纤维细胞,
Key words：fibroblast growth factor,periodontal regeneration,fibroblasts,
基金项目：山东省自然科学基金资助项目(993205806)
中文摘要
目的 观察碱性成纤维细胞生长因子(bFGF)对犬牙周成纤维细胞样细胞增殖的影响。方法 选用4只成年杂种犬,将其上颌两侧的前磨牙(P1-3)、第一磨牙(M1)、下颌两侧的前磨牙(P2-4)、第一磨牙(M1)作为实验牙。在每个象限4个实验牙的近中根颊侧人工制备“U”形骨缺损,并设计为4组,即对照组、bFGF组、ePTFE组、bFGF+ ePTFE组。每周手术1次,每次手术做1个象限的4个牙位,连续4次。完成4次手术后1周处死动物。动物处死前1 h注射尿嘧啶脱氧核苷(BrdU),标本行免疫组化染色,显微镜下计数BrdU标记的成纤维细胞样细胞。结果 各组中BrdU+细胞数均于术后2周最多,术后3、4周显著减少。术后1、2周,bFGF组和bFGF+ePTFE组的BrdU+细胞明显多于对照组和ePTFE组。结论 牙周术后2周是牙周功能细胞增殖的活跃期,bFGF的局部应用可明显促进牙周愈合早期牙周功能细胞的增殖,从而促进牙周再生。
英文摘要
Objective To evaluate the effects of basic fibroblast growth factor(bFGF) on proliferation of periodontal fibroblast- like cellsin vivo.Methods AU-shaped osseous defectwas produced on the buccal side of the mesial root. Four posterior teeth were conducted in four quadrants. Each quadrant included 4 groups: control, bFGF, expanded polytetrafluoroethylene(ePTFE) membrane, bFGF+ePTFE. Each time the 4 teeth sites in one quadrantwere operatedweekly and each dog experienced 4 times of operations. Bromodeoxyuridine(BrdU) was injected 1 hour priorto sacrificingthe dogs at 4 weeks afterfirst surgery. Immunohisto- chemical method was applied to count the BrdU-labeled fibroblast-like cells.Results The number of BrdU-labeled cells reached the maximum at the 2nd week among all groups and then, decreased with time. Both bFGF and bFGF+ePTFE treated group had significantly more BrdU+cells than remained control or ePTFE groups (P
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