成骨细胞在快速成型技术构建的骨支架材料中粘附的实验研究
Experimental Study of Periosteal Osteoblasts Adhesion to Artificial Bone Scaffolds Based on Rapid Prototype
作者：鲜苏琴,柴枫,赵铱民,王忠义,刘小芳,李涤尘
Author：XIANSu- qin1,CHAI Feng2, ZHAO Yi-min2, WANG Zhong-yi2,LIUXiao-fang2, LITiao-cheng3
收稿日期：2004-06-25 年卷（期）页码：2004,22(03):248-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：骨组织工程,快速成型技术,细胞相容性,成骨细胞,
Key words：bone tissue engineering,osteoblast adhesion,rapid prototype,cellular biocompatibility,
基金项目：国家自然科学基金资助项目(59975074)
中文摘要
目的 观察成骨细胞在用快速成型技术构建的具有不同孔隙率的骨支架材料中的粘附情况以及材料的孔隙率对成骨细胞粘附的影响,以探索用快速成型技术构建骨组织工程支架材料的可行性。方法 体外分离培养、扩增乳鼠颅骨骨膜成骨细胞,经传代培养作为种子细胞,接种于由快速成型技术构建,孔隙率分别为80%、 90%、95%的骨支架材料中,复合培养4 d及10 d后,采用细胞计数及扫描电镜方法检测支架材料中细胞粘附的情况。结果 ①各孔隙率组粘附成骨细胞总数均呈现随培养时间延长而增高的趋势(P
英文摘要
Objective To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.Methods Infant rat calvarias osteoblastswere isolated and expandedin vitroand the cells (2nd passage) were seeded onto scaffolds with porosity 80%,90%,95% at a density of 2·06×109/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.Results ①The osteoblasts′adhesion amounts increased with culture time in three porosity group (P
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