nm23-h1转染腺样囊性癌细胞及其增殖能力的实验研究

Objective To study the effect of nm23-h1 transfection on proliferation characteristics of adnoid carcinoma cell linesin vitroandin vivo.Methods In vitroACC-M cell lines were incubated after putative anti-metastatic gene nm23-h1 was introduced into the cells with the help of G418 selective incubation base. The ACC-M cells were transplanted into 10 BALB/C nude mices subcutaneously and non-transfected cell lines were taken as control. Immunohisto chemistry and Ki67 antibody were employed to study the proliferation character of cell crawling pieces and paraffin-bedded slice, meanwhile, the solid tumor of both groups were prepared for flow cytometry(FCM).Results Transfected cells grew slower than non-transfected cells and this trend became more obvious as passages passed on.In vitrothe expression of Ki67 of transfected cells was little stronger than non-trans- fected cells, while the expression of Ki67 in solid slices was almost negative in both groups. Transfected cells presented slower growth than non-transfected cells in the early stage(2 weeks) and 2 weeks later there was no obvious difference in size(P> 0·05). FCMvalue accordedwellwith the result.Conclusion Introduction of nm23-h1 into the ACC-M cell lines may have tran- sient inhibitory effects on its proliferation.