肿瘤坏死因子α和白细胞介素2在大鼠皮肤移植中的作用
Study on Tumor Necrosis Factor-αand Interleukin 2 in Rat Skin Allograft
作者：王军琳,金岩,刘晓亮
Author：WANGJunlin,JINYan,LIUXiaoliang.
收稿日期：2003-12-25 年卷（期）页码：2003,21(06):471-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：肿瘤坏死因子α,白细胞介素2,组织工程,同种异体移植,
Key words：tumor necrosis factor-α,interleukin-2,tissue engineering,allograft,
基金项目：
本课题为国家科技部“863”重大专项课题资助项目(编号 002AA205041)
中文摘要
目的 探讨肿瘤坏死因子α(TNF-α)和白细胞介素2(IL-2)在同种异体大鼠皮肤移植后免疫排斥反应中的作用,研究组织工程皮肤的组织相容性。方法 取SD大鼠皮肤和实验室培养的组织工程SD大鼠皮肤移植于成年Wistar大鼠,移植成功后取不同时间点标本用免疫组织化学方法和Western blot方法检测TNF-α和IL-2在组织中的表达。结果 同种异体皮肤移植组,TNF-α和IL-2有显著表达;组织工程皮肤移植后,局部组织中TNF-α和IL-2 水平显著低于同种异体皮肤移植组。结论 TNF-α和IL-2水平的改变与同种异体皮肤移植后免疫排斥反应的发生密切相关,组织工程皮肤只具有很弱的免疫原性,不引起显著的排斥反应。
英文摘要
Objective To elucidate the histocompatibility of tissue engineered rat skin through studying the effect ofTNF- αand IL-2 in immunological rejection after rat skin allograft.Methods Tissue engineered skin thatthe basic materialswere taken from neonatal SD rats was cultured in lab, grafted to adult Wistar rats. The expressions of TNF-αand IL-2 in grafted tissue were detected with immunohistochemical staining and Western blot.Results The expressions of TNF-αand IL-2 were remarkable in skin allograft group, but low in tissue engineered skin group.Conclusion The expressions of TNF-αand IL-2 and the immuno- logical rejection were closely related after skin allograft, but the tissue engineered skin has favorable histocompatibility and doesn′t arose obvious immunological rejections.
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