葡糖基转移酶多肽疫苗HDS免疫大鼠的实验研究
The Study of Peptide Vaccine HDS fromStreptococcus mutansGlucosyltransferase
作者：丁芸,凌均棨,陈罕
Author：DING Yun,LINGJunqi,CHENHan
收稿日期：2003-10-25 年卷（期）页码：2003,21(05):353-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：龋病,变形链球菌,葡糖基转移酶,多肽,疫苗,
Key words：caries,Streptococcus mutans,glycosyltransferase,vaccine,
基金项目：本课题为广东省自然科学基金资助项目(编号001417)
中文摘要
目的 动物体内研究葡糖基转移酶多肽疫苗HDS免疫原性和防龋效果。方法 构建大鼠人工龋模型,分别用人工抗原HDS-KLH,多肽抗原HDS,葡糖基转移酶(GTF)免疫大鼠,酶联免疫吸附法检测大鼠血清和唾液中抗HDS、GTF抗体;体视显微镜下观察各组大鼠磨牙龋坏情况,并用Keyes计分法计分。结果 HDS-KLH免疫组大鼠唾液、血清中抗HDS IgG和IgA水平均高于对照组(P
英文摘要
Objective To investigate the antigenicity of the peptide vaccineHDS fromStreptococcus mutansglucosyltrans- ferase and its ability to induce protective immune responses in an experimental ratmodel of dental caries.Methods Artificial an- tigenHDS-KLH, peptide HDS, glycosyltransferase were injected to immunize rats. Measurement of the specific anti-HDS,GTF IgG or IgA concentration in saliva and serumwere undertaken by ELISAamongthe experimental groups. Gnotobiotic ratmodelwas developed when challengedS.mutansand a caries promoting diet. The jaws of the ratswere selected and dyed. The Keyes caries score for each jawwere counted.Results The level of serum and salivary specific anti-HDS IgG and IgA in the group immunized by HDS-KLHwas significantly higher than that in control group (P
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