热疗对肿瘤细胞耐药性的影响
Reversal Effect of Hyperthemia on Multidrug Resistant Phenomena
作者：张萍,王大章,郑光勇,刘曙光
Author：ZHANG Ping,WANG Dazhang,ZHENG Guangyong,et al.
收稿日期：2003-04-25 年卷（期）页码：2003,21(02):127-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：肿瘤,多药耐药,化疗,热疗,
Key words：tumor,multidrug resistance,chemotherapy,hyperthemia,
基金项目：本课题为国家自然科学基金资助项目(编号39570768)
中文摘要
目的 探讨热疗对肿瘤细胞耐药性的产生和变化的影响。方法 采用RT-PCR法比较41℃及37℃ 下Tca8113及K562/ADM细胞株在体外接受短期化疗后,在mRNA水平上多药耐药基因(MDR1)及多药耐药相关蛋白基因(MRP)的表达情况,采用荧光分光光度计检测细胞内药物浓度的变化。结果 热疗化疗联合应用对Tca8113 及K562/ADM细胞株抑制作用明显提高;41℃下热疗可在mRNA水平部分逆转K562/ADM细胞中MDR1和MRP的表达,并明显升高了K562/ADM细胞内药物浓度。结论 热疗不仅抑制肿瘤细胞耐药基因的表达,同时还有增加细胞内的药物浓度、拮抗耐药蛋白的作用,从而逆转耐药。
英文摘要
Objective The purpose of this study was investigate the effect of hyperthemia on multidrug resistance in K562/ADM cell.Methods The MDR1 (mulitdrug resistance gene) andMRP (multidrug resistant associated gene) gene expres- sions in Tca8113 and K562/ADM cell lineswere analyzed by RT-PCR aftertreatedwith different cytotoxic drugs and different tem- perature (37℃and 41℃). The function and expression of Pgp andMRPwere detected by fluorescence photometeric assays.Re- sults Inhibition rate of both cellswas significantly enhanced by exposure to chemotherapeutic drugs and 41℃temperature; Ex- posing to 41℃hyperthemia reduced MDR1 and MRP expression and enhanced intracellular drug concentration as well in K562/ ADM.Conclusion 41℃hyperthemia could effectively enhance the inhibition rate of chemotherapeutic drugs and partially re- verse the multidrug resistance. It is suggested that hyperthemia could be used as a method to overcome multidrug resistance.
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