ICAM-1和LFA-1单抗对口腔鳞癌肿瘤浸润淋巴细胞杀伤活性的影响
The Effect of Anti-ICAM-1/LAF-1 McAb on the Cytotoxicity of Tumor Infiltrating Lymphocytes from Oral Squamous Cell Carcinoma
作者：杨宏宇,罗娟,李金荣,周军
Author：YANGHongyu*,LUOJuan,LIJinrong,et al.
收稿日期：2003-04-25 年卷（期）页码：2003,21(02):107-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：癌,鳞状细胞,T淋巴细胞,细胞毒性,粘附分子,
Key words：carcinoma,squamous cell,T-lymphocyte,cytotoxicity,adhesive molecular,
基金项目：
中文摘要
目的 研究ICAM-1/LFA-1单抗阻断对口腔鳞癌肿瘤浸润淋巴细胞(TIL)对自体肿瘤细胞杀伤活性及分泌TNF-α的影响。方法 从15例口腔鳞癌组织中分离TIL并培养,用流式细胞术检测新鲜分离和激活的TIL细胞上ICAM-1和LFA-1的表达水平;比较用ICAM-1/LFA-1单抗阻断前后TIL对自体肿瘤细胞杀伤活性及产生TNF-α 水平的影响。结果 激活的TIL较新鲜分离的TIL细胞ICAM-1和LAF-1的表达显著增加(P0·05),但TIL 对自体肿瘤细胞的杀伤活性显著降低(P
英文摘要
Objective To investigate the effect of anti-ICAM-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lym- phocytes from oral squamous cell carcinoma.Methods TILwere isolated from fresh tumor tissues in 15 patients with oral squa- mous cell carcinoma and culturedin vitro. The expression of ICAM-1/LAF-1 in fresh and activated TILwas examined by FACS. The cytotoxicity of TIL against auto tumor cells and the level ofTNF-αproduced byTILwere compared before and after interrupted with anti- ICAM-1/LAF-1 McAb.Results The expression of ICAM-1and LAF-1 in the activatedTIL significantly increased com- paring with freshTIL (P
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