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论文摘要

PTEN基因转染对粘液表皮样癌细胞系M_3SP_2增殖的抑制作用

Transfection of the Exogenous PTEN-induced Growth Inhibition of the Highly Metastatic Mucoepidermoid Carcinoma Cell Line M3SP2in vitro

作者:刘斌,吴军正,孙安,李焰,叶维莉

Author:Liu Bin, Wu Junzheng, Li Yan, et al

收稿日期:2002-10-25          年卷(期)页码:2002,20(05):361-

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:抑癌基因,粘液表皮样癌,基因转染,细胞生长,

Key words:phosphatase and tensin homology deleted on chromosome 10,mucoepidermoid carcinoma,gene transfection,

基金项目:

本课题为陕西省自然科学基金(编号99SM32)和第四军医大学创新工程(编号CX99A011)资助项目

中文摘要

目的:观察外源磷酸酶和张力蛋白同源物(PTEN)抑癌基因对高转移性粘液表皮样癌细胞系M3SP2体外生长的影响。方法:应用脂质体介导方法将野生型PTEN基因导入M3SP2细胞,通过活细胞观察、细胞生长曲线、分裂指数和克隆形成率了解细胞生长状态。结果:与亲本细胞比较,PTEN基因转染细胞数量减少、排列松散,部分细胞发生变性或崩解;细胞生长缓慢,分裂指数显著减至1612j(P

英文摘要

Objective:The purpose of this study was to evaluate the effects of the exogenous phosphatase and tensin homology deleted on chromosome 10 (PTEN) gene on in vitro growth of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.Methods: The growth of the exogenous PTEN transfected mucoepidermoid carcinoma cells M3SP2-PTEN gene was studied by analyzing cell growth curves, mitosis index and clone formation efficiency and compared with its parental cell line M3SP2and the vector pBabe- puro-transfected cell line M3SP2-pBp.Results:The doubling time (h) ofM3SP2, M3SP2-pBp andM3SP2-PTENwere 24.50, 24. 76 and 31.74; the mitosis index(j) were 53.0?6.20, 49.0?5.24 and 16.2?3.2; the clone formation efficiency (%) were 37.37, 35.01 and 10.40, respectively. The M3SP2-PTEN cells also revealed 57.05%~71.46% inhibition of growth from day 3 to7 and 65%~72% inhibition of clone formation compared with the parental cells.Conclusion:These data provide evidence that the exogenous wild-type PTEN have remarkably inhibitory effects on in vitro proliferation of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.

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