人口腔鳞癌细胞系对淋巴靶向葫芦素BE聚乳酸纳米微粒的敏感性研究
Effects of Cucurbitacin BE Polylactic Acid Nanoparticles on Human Oral Squamous Carcinoma Cell Lines
作者：杨凯,王昌美,温玉明,李龙江,王晓毅,陈绍维
Author：Yang Kai, Wang Changmei, Wen Yuming, Li Longjiang, et al
收稿日期：2002-08-25 年卷（期）页码：2002,20(04):293-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：葫芦素BE,聚乳酸,钠米微粒,口腔癌,颈淋巴结转移,靶向化疗,四唑盐显色法,
Key words：cucurbitacinBE polylactic acid,nanoparticles,oral carcinoma,cervical lymph node metastasis,targeted chemotherapy,MTT assay,
基金项目：
本课题为卫生部科学基金(编号98-1-221)及重庆医科大学附属第一医院科研启动基金(编号2002007)资助项目
中文摘要
目的:探讨人口腔鳞癌细胞系(Tca8113和BcaCD885)对颈淋巴结靶向性葫芦素BE聚乳酸纳米微粒(CuBE- PLA-NP)的敏感性。方法:用四唑盐显色法(MTT法)检测用不同剂量的CuBE-PLA-NP和CuBE在1~8 d内8个时间点对Tca8113和BcaCD885的抗癌活性,计算出2种药物在8个时间点对2种癌细胞的抑癌率和药物的半数抑癌率浓度。结果:CuBE-PLA-NP和CuBE对Tca8113和BcaCD885均有强的杀伤作用,其杀伤效应均呈时间依赖性和剂量依赖性。结论:经改型后的淋巴靶向CuBE-PLA-NP没有降低CuBE成份的抗癌活性,同时,由于CuBE-PLA-NP具有淋巴靶向性,更有利于杀灭口腔癌颈淋巴结转移灶内的癌细胞。
英文摘要
Objective:The aim of this study was to study cytotoxic effects of cucurbitacin BE polylactic acid nanoparticles (CuBE-PLA- NP) on human oral squamous carcinoma cell lines Tca8113 (tongue carcinoma) and BcaCD885 (buccal carcinoma).Methods: The chemosensitivity of Tca8113 and BcaCD885 to CuBE-PLA-NP and CuBEwas tested usingMTTassay, the inhibitory rate and 50% inhibition concentrations values (IC-50) of these two drugs against these two cancer cell lineswere evaluated in 1,2,3,4,5, 6,7 and 8 days with different concentrations.Results:Both CuBE-PLA-NP and CuBE showed very high anti-cancer effects on Tca8113 and BcaCD855, and the anticancer efficacy of these two drugswas dose- and time-dependent.Conclusion:The effects of CuBE-PLA-NP on lymph nodes of metastatic cancer cells were higher than that of CuBE.
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