抗人血管内皮生长因子单克隆抗体抑制人颊鳞癌生长的实验研究
An Experimental Study on the Inhibitory Effects of Anti-human Vascular Endothelial Growth Factors on the Growth of Buccal Mucosa Carcinoma
作者：房思炼,王大章,杨西川,李虹,郑光勇,张静仪
Author：Fang Silian, Wang Dazhang,Yang Xichuan, Li Hong, et al
收稿日期：2002-04-25 年卷（期）页码：2002,20(02):89-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：血管内皮生长因子,单克隆抗体,肿瘤生长,血管生成,动物模型,
Key words：vascular endothelial growth factor,tumor growth,angiogenesis,monoclonal antibodies,animal models,
基金项目：本课题为国家自然科学基金资助项目(编号39970798)
中文摘要
目的:研究抗人血管内皮生长因子(VEGF)单抗E11抑制移植性人颊鳞癌血管生成,从而抑制颊癌生长的作用效果。方法:接种人颊鳞癌BCa CD885细胞株的BALBPc裸小鼠分别通过腹腔或瘤周皮下注射抗人VEGF单抗或生理盐水。每隔3 d测量瘤体积1次,接种后第18天处死裸鼠并称取瘤重,计算抑瘤率。结果:¹不同给药途径的抗人VEGF单抗对颊癌的生长均有明显的抑制作用,与生理盐水组相比较有显著性差异,其抑瘤率分别为6011%、 6919%。º腹腔、瘤周皮下两种注射方式比较,瘤周皮下组的抑瘤效果更为显著。结论:肿瘤中的VEGF是抗肿瘤血管生成的理想靶目标,而抗人VEGF单抗又是该抑癌新途径的理想选择,具有潜在的重要临床应用价值。
英文摘要
Objective:The purpose of this study was to apply the anti-human VEGF monoclonal antibody E11, which was generated against a synthetic peptide from the NH2of the human VEGF (residues 1-26), to againstVEGF in buccal carcinoma, and to ex- amine the inhibition effect of the antibody on the carcinoma growth.Methods:E11was hypodermic and celiac injected, with 200 Lg each from the first to the 15th day, into BALBPc nuPnu mice, whichwere transplanted with human buccal carcinoma. The sa- line was injected as the negative control. Mice were killed on the 18th day. The tumor weight was determined and the inhibition rate was calculated.Results:The growth of BCaCD885 carcinomawas dramatically inhibited in the E11group. There was a signifi- cant difference in tumorweight between the saline group and the E11groups during the observation period. The tumor growth inhi- bition rate in the mice hypodermically injected with E11reached 69.9%.Conclusion:The anti-human VEGFmonoclonal antibody E11significantly inhibits tumor growth by blocking the action of VEGF, and without obvious side effects, therefore the anti-human VEGF monoclonal antibody could be applied clinically in the anti-angiogenic treatment of solid tumors.
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