程序性死亡分子1及其配体在口腔鳞状细胞癌患者外周血中的表达及临床意义
Levels of programmed death-1 and programmed death ligand-1 in the peripheral blood of patients with oral squamous cell carcinoma and its clinical implications
作者：张鹏 欧阳少波 王军 黄自坤 王娇龙 廖岚
Author：Zhang Peng, Ouyang Shaobo, Wang Jun, Huang Zikun, Wang Jiaolong, Liao Lan.
收稿日期：2015-02-15 年卷（期）页码：2015,33(5):529-529-533
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：口腔鳞状细胞癌,T淋巴细胞,程序性死亡分子1,程序性死亡分子配体1,
Key words：oral squamous cell carcinoma,T-lymphocyte,programmed death-1,programmed death ligand-1,
基金项目：
江西省教育厅科学技术研究基金资助项目（GJJ14052）
中文摘要
目的 检测口腔鳞状细胞癌患者外周血中程序性死亡分子1（PD-1）及其配体（PD-L1）的表达水平，探讨其生物学及临床意义。方法 选取82例口腔鳞状细胞癌患者（口腔鳞癌组）和25例健康对照者（对照组）为研究对象，收集研究对象的外周血，采用流式细胞术检测外周血T淋巴细胞表面PD-1、PD-L1的表达及T淋巴细胞亚群计数，采用酶联免疫吸附方法检测血清中可溶性PD-1（sPD-1）和可溶性PD-L1（sPD-L1）的表达水平。采用SPSS 17.0软件对数据进行检验和相关性分析。结果 口腔鳞癌组外周血CD8+ T淋巴细胞百分数明显高于对照组（P0.05）。sPD-L1的表达与临床分期、肿瘤分化程度及淋巴结转移状态相关（P结论 口腔鳞状细胞癌患者外周血T淋巴细胞亚群存在不同程度的免疫功能抑制，CD4+和CD8+ T淋巴细胞表面PD-1及PD-L1表达显著升高。异常升高的sPD-L1可能与口腔鳞状细胞癌的发生发展有关。
英文摘要
Objective To detect the expression levels of programmed death-1 (PD-1) and programmed death ligand-1 (PD-L1) in the peripheral blood of patients with oral squamous cell carcinoma (OSCC) and to discuss their biological and clinical significance.Methods PD-1/PD-L1 expression on the surface of T-lymphocytes and the counts of T-lymphocyte subpopulations of peripheral blood in 82 patients with OSCC (OSCC group) and 25 healthy controls (control group) were examined via flow cytometry. The expression levels of soluble PD-1 (sPD-1) and soluble PD-L1 (sPD-L1) in the serum were observed through enzyme-link immunology method. The data were tested and analyzed with SPSS 17.0 software.Results The percentage of CD8+ T cells in the OSCC group was significantly higher than that in the control group (P0.05), but the average of sPD-L1 was remarkably higher than that in the control group (PConclusion T-lymphocyte subpopulations in the peripheral blood of patients with OSCC developed immunosuppression with different degrees. PD-1 and PD-L1 expression levels on the surface of CD3+ and CD4+ T cells significantly increased. Abnormal increase in sPD-L1 expression may be associated with OSCC development.
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