白细胞介素-23通过无翅基因相关整合位点/β-连环蛋白通路增强舌鳞状细胞癌抗凋亡及耐药能力
Interleukin-23 strengthens the anti-apoptotic and drug resistance of human tongue squamous cell carcinoma through the Wingless-related integration site/β-catenin pathway
作者：严嵚 苏玉婷 周月鹏 朱海涛 杨细虎 许建辉
Author：Yan Qin, Su Yuting, Zhou Yuepeng, Zhu Haitao, Yang Xihu, Xu Jianhui.
收稿日期： 年卷（期）页码：2015,33(3):249-249-254
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：白细胞介素-23, 舌鳞状细胞癌, 耐药, 抗凋亡, 无翅基因相关整合位点,
Key words：interleukin-23; ,tongue squamous cell carcinoma; ,drug resistance,anti-apoptosis; ,Wingless-related integration site,
基金项目：
中文摘要
目的 检测白细胞介素-23（IL-23）在舌鳞状细胞癌组织中表达水平与临床预后的关系，研究舌鳞状细胞癌细胞系SCC9经IL-23处理后抗凋亡及耐药能力的变化。方法 免疫组织化学法检测28例舌鳞状细胞癌患者组织中IL-23的表达情况；实时定量聚合酶链反应（qRT-PCR）检测不同浓度的IL-23处理后，SCC9中无翅基因相关整合位点（Wnt）1及c-myc的mRNA表达水平；结合小分子干扰RNA（siRNA），Western blot法检测IL-23对SCC9细胞的β-连环蛋白（β-catenin）和B淋巴细胞瘤-2（Bcl-2）、三磷酸腺苷结合转运蛋白G超家族成员2（ABCG2）、P-糖蛋白（P-gp）表达影响及潜在机制；甲基噻唑基四唑法检测SCC9细胞在IL-23作用下对顺铂的抗凋亡能力改变。结果 舌鳞状细胞癌组织中IL-23表达强度与淋巴结转移、神经侵犯及治疗复发有相关性（PP结论 IL-23通过上调舌鳞状细胞癌细胞的Wnt通路增强其抗凋亡及耐药能力。
英文摘要
Objective This study aims to detect the expression level of interleukin-23 (IL-23) in tongue squamous cell carcinoma tissues and its relationship with clinical prognosis, as well as explore the anti-apoptotic and drug resistance of the tongue squamous cell line-SCC9 before and after treatment with IL-23.Methods The expression of IL-23 in tumor tissues from 28 tongue cancer patients was analyzed by immunohistochemistry assay. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression of Wingless-related integration site (Wnt)1 and c-myc in SCC9 cells treated with different IL-23 concentrations. After interferencing the β-catenin with small interfering RNA (siRNA), the expression of β-catenin, B-cell lymphoma-2 (Bcl-2), ATP-binding cassette sub-family G member 2 (ABCG2), and permeabilityglycoprotein (P-gp) in SCC9 was measured by Western blot analysis. The effect of IL-23 on the apoptotic resistance of SCC9 to cisplatin was examined by methyl thiazolyl tetrazolium test.Results The expression of IL-23 in tongue cancer tissues was correlated with lymphatic metastasis, nerve invasion, and the recurrence after therapy (PPConclusion IL-23 activates the Wnt pathway in tongue squamous cell carcinoma, thereby enhancing its resistance to apoptosis and drug.
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