转化生长因子-β1和白细胞介素-10单核苷酸多态性与复发性口腔溃疡易感性的研究
Relationship between transforming growth factor-β1 and interleukin-10 single nucleotide polymorphism and susceptibility of recurrent aphthous ulcer
作者：张敬 沙晶晶 龚娟
Author：Zhang Jing, Sha Jingjing, Gong Juan
收稿日期： 年卷（期）页码：2016,34(1):27-27-31
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：转化生长因子-&beta,1, 白细胞介素-10, 单核苷酸多态性, 复发性口腔溃疡,
Key words：transforming growth factor-β1, interleukin-10, single nucleotide polymorphism,recurrent aphthous ulcer,
基金项目：
宁夏自然科学基金（NZ201322）
中文摘要
目的 研究转化生长因子-β1（TGF-β1）-509T/C位点与白细胞介素-10（IL-10）-1082A/G位点的单核苷酸多态性与复发性口腔溃疡（RAU）易感性的相关性。方法 采用限制性片段长度多态性-聚合酶链式反应（RFLPPCR）RFLPPCR）法和序列特异性引物-聚合酶链式反应（SSP-PCR）法对138例RAU患者和124例健康对照者进行TGF-β1-509位点与IL-10-1082位点的单核苷酸多态性的检测分析，用比值比（OR）和95%可信区间（95%CI）估计相对危险度。结果 TGF-β1-509位点与IL-10-1082位点在基因型频率与等位基因频率的分布上，病例组与正常对照组之间均存在明显差异（P结论 TGF-β1-509位点与IL-10-1082位点是RAU患者的易感基因位点。TGF-β1-509位点携带T等位基因患RAU的风险性是携带C等位基因者的1.465倍。IL-10-1082位点携带G等位基因患RAU的风险性是携带A等位基因者的2.134倍。
英文摘要
Objective To explore the possible relationship between recurrent aphthous ulcer (RAU) and single nucleotide polymorphism (SNP) of transforming growth factor-β1 (TGF-β1)-509T/C and interleukin-10 (IL-10)-1082A/G sites.Methods A total of 138 RAU patients were recruited for this study. The control group consisted of 124 subjects. TGF-β1-509T/C and IL-10-1082A/G sites were detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) and sequence specific primer-polymerase chain reaction (SSP-PCR). Relative risk ratios were estimated by odds ratios (OR) and 95% confidence interval (95%CI).Results Significant differences were found in the genotype frequencies or allele frequencies of TGF-β1-509T/C and IL-10-1082A/G sites between the RAU patients and controls (PConclusion TGF-β1-509T/C and IL-10-1082A/G sites are associated with the risk of RAU. The TGF-β1 gene-509T allele and IL-10 gene-1082G allele may serve as genetic determinants for RAU.
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