体外沉默异戊二烯化酶二牛龙牛儿基转移酶Ⅰ对舌鳞状细胞癌增殖的影响
Effects of geranylgeranyltransferaseⅠsilencing on the proliferation of tongue squamous cancer cells
作者：王莹, 王奇民, 李敬华, 韩金宏, 王莉莉, 巢晨, 周建华, 童磊, 鲁旭飞, 周元, 廖奕翔, 何宗轩, 李宁, 曹蕾, 刘文君, 陈正岗
Author：Ying Wang, Qimin Wang, Jinghua Li, Jinhong Han, Lili Wang, Chen Chao, Jianhua Zhou, Lei Tong, Xufei Lu, Yuan Zhou, Yixiang Liao, Zongxuan He, Ning Li, Lei Cao, Wenjun Liu, Zhenggang Chen
收稿日期：2016-10-11 年卷（期）页码：2017,35(4):373-373-378
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：异戊二烯化酶二牛龙牛儿基转移酶Ⅰ,RhoA,舌鳞状细胞癌,增殖,Cyclin D1,p21,
Key words：geranylgeranyltransferaseⅠ,RhoA,tongue squamous cancer,proliferation,Cyclin D1,p21,
基金项目：国家自然科学基金（81372908）;青岛市卫计委计划项目（2014-WJZD009,2013-WSZD011）
中文摘要
目的 研究异戊二烯化酶二牛龙牛儿基转移酶Ⅰ（GGTase-Ⅰ）在舌鳞状细胞癌增殖中的作用。方法 登录Genebank确定人GGTase-Ⅰ基因序列,设计3条小干扰RNA（siRNA）,并将siRNA转染至舌癌细胞Cal-27（GGTase-ⅠsiRNA组）。设立空白对照组（只加入转染试剂,不加入siRNA）和阴性对照组（NC-siRNA）。采用实时定量聚合酶链反应和蛋白质免疫检测转染后各组细胞GGTase-Ⅰ、RhoA的mRNA和蛋白表达;蛋白质免疫检测转染48 h后Cyclin D1、p21的表达变化;细胞增殖活性检测试剂盒和流式细胞术检测细胞的增殖活性和细胞周期变化。结果 与阴性对照组和空白对照组相比,GGTase-Ⅰ siRNA 组细胞的GGTase-Ⅰ的mRNA和蛋白表达下降（P<0.05）,RhoA的mRNA和蛋白表达无明显改变（P>0.05）;Cyclin D1的表达下降,p21表达升高,细胞的增殖活性下降,细胞周期发生改变（P<0.05）。结论 GGTase-Ⅰ siRNA能抑制舌鳞状细胞癌细胞中GGTase-Ⅰ的表达,抑制细胞增殖,提示GGTase-Ⅰ在舌鳞状细胞癌增殖中可能发挥重要作用。
英文摘要
ObjectiveThis study aims to investigate the effect of geranylgeranyltransferaseⅠ (GGTase-Ⅰ) on the proliferation and growth of tongue squamous cancer cells.MethodsThree small interfering RNAs (siRNAs) were designed on the basis of the GGTase-Ⅰ sequence in GeneBank. These siRNAs were then transfected into tongue squamous cancer cells Cal-27. The mRNA and protein expression of GGTase-Ⅰ and RhoA were examined by real-time quantitative polymerase chain reaction and Western blotting, respectively. The expression of Cyclin D1 and p21 were examined by Western blotting. The proliferation and growth ability were analyzed by cell counting kit-8 assay and flow cytometry.ResultsThe mRNA and protein expression of GGTase-Ⅰ in Cal-27 was reduced significantly after the GGTase-Ⅰ siRNAs were transfected (P<0 .05). no significant difference in rhoa mrna and protein expression was detected (P>0.05). Cyclin D1 expression decreased, whereas p21 expression increased significantly. The cell cycle was altered, and the growth-proliferative activity was inhibited (P<0 .05).ConclusionGGTase-Ⅰ siRNA can inhibit the expression of GGTase-Ⅰ and the proliferative activity of tongue squamous cancer cells. GGTase-Ⅰ may be a potential target for gene therapy in tongue squamous cell cancer.
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