局部注射外源性神经生长因子促进小鼠钛种植体周骨胶原早期成熟的研究
Local injection of exogenous nerve growth factor improves early bone maturation of implants
作者：姚洋, 杜宇, 古霞, 光梦凯, 黄波, 宫苹
Author：Yang Yao, Yu Du, Xia Gu, Mengkai Guang, Bo Huang, Ping. Gong
收稿日期：2017-09-22 年卷（期）页码：2018,36(2):128-128-132
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：神经生长因子,钛种植体,骨整合,
Key words：nerve growth factor,titanium implant,osseointegration,
基金项目：国家自然科学基金（81170995）;四川大学华西口腔医院青年科学研究基金（WCHS-201514）;四川大学青年教师科研启动基金（2016SCU11057）
中文摘要
目的 探讨神经生长因子（NGF）对骨整合早期种植体周骨组织的影响。方法 在小鼠腿骨植入钛种植体区局部注射外源性NGF,建立小鼠腿骨钛种植体-NGF模型。并于术后1、2、4周分别采用苏木精-伊红（HE）和Masson染色,探讨NGF在种植体骨整合早期不同时间点对种植体周骨胶原成熟的影响。结果 HE染色与Masson染色显示,体内局部注射NGF使小鼠股骨头钛种植体模型中种植体周骨量、骨胶原成熟度增加。术后第1周和第4周实验组成熟骨胶原占比均高于对照组（P<0.05）。结论 体内局部注射NGF能加速小鼠腿骨内钛种植体周新生骨胶原的早期成熟。
英文摘要
ObjectiveTo investigate the effects of nerve growth factor (NGF) in the osteogenic action of implants and the maturation and reconstruction changes in bone tissues in the early stage of osseointegration.MethodsThe mouse implant model was established by placing titanium in the femoral head of the mouse and locally injecting NGF in the implant zone. On 1, 2 and 4 weeks after operation, stain samples were collected from animals using hematoxylin-eosin (HE) staining and Masson staining. The effect of NGF on the bone maturation was compared at different time points of early stage osseointe-gration.ResultsThe results of HE and Masson staining indicated that the local injection of external NGF can up-regulate bone mass, amount of bone trabecula, and bone maturity in the mouse model. The mature bone rate in treatment group of 1 week and 4 weeks after operation were significantly higher than those in the control group (P<0 .05).ConclusionNGF can shorten the period of bone maturation.
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