调节Lnk/干细胞因子-干细胞因子受体通路对糖尿病状态骨髓间充质干细胞成骨功能的影响

Objective Changes in the osteogenesis of diabetic rat bone marrow mesenchymal stem cells (BMSCs) by the regulation of Lnk/stem cell factor (SCF)-cKit signaling were investigated. Methods BMSCs were isolated from diabetic rats and identified by immunocytochemical staining. These cells were divided into the control group (untransfected group), negative control group (transfected with control plasmid), and RNA interference group (transfected with Lnk-targeting RNA interference plasmid). Western blot was performed to analyze the effect of interference. The BMSCs were induced for osteogenic differentiation under diabetic conditions, and Western blot was used to examine the expressions of Lnk, SCF, and cKit in Lnk/SCFcKit signaling and osteogenic proteins alkaline phosphatase (ALP), osteocalcin (OCN), and collagen type Ⅰ a1 (ColIa1). Results Isolated cells expressed CD₄₄and CD₉₀but not CD_11bor CD₄₅. This phenomenon was characteristic of BMSCs. Compared with other diabetic BMSCs, cells in the RNA interference group expressed low Lnk but high SCF and cKit (PP